{"product_id":"nolc1-antibody-nucleolar-and-coiled-body-phosphoprotein-1-nopp140-bha17135743","title":"NOLC1 Antibody \/ Nucleolar and coiled-body phosphoprotein 1 \/ NOPP140","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNOLC1 Antibody \/ Nucleolar and coiled-body phosphoprotein 1 \/ NOPP140 is a anti-NOLC1 Rabbit antibody Recombinant Rabbit Monoclonal clone 30N31 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NOLC1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30N31, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNOLC1 antibody detects nucleolar and coiled-body phosphoprotein 1, encoded by the NOLC1 gene. This protein is also called NOPP140, nuclear phosphoprotein 140, and hNOPP140. NOLC1 is a nucleolar protein that shuttles between the nucleolus and cytoplasm, functioning as a phosphoprotein involved in ribosome biogenesis, rRNA transcription, and nucleolar organization. Structurally, NOLC1 contains multiple serine-rich regions that undergo phosphorylation, regulating its localization and interactions with RNA polymerase I and ribosomal proteins.\u003cbr\u003e\u003cbr\u003eNOLC1 antibody is widely applied in cell biology, cancer research, and nucleolar studies. By detecting NOLC1, researchers can investigate how the nucleolus coordinates ribosome production and stress responses. The protein is highly expressed in proliferating cells, linking it to growth and transformation. Dysregulated NOLC1 expression has been reported in cancers, where nucleolar function is often enhanced to meet elevated translational demands.\u003cbr\u003e\u003cbr\u003eApplications of NOLC1 antibody include western blotting, immunohistochemistry, immunofluorescence, and ELISA. Western blot assays detect phosphorylated and unphosphorylated isoforms, immunohistochemistry maps expression in tumors, and immunofluorescence highlights nucleolar localization and dynamics. These approaches provide powerful tools for studying nucleolar biology in health and disease.\u003cbr\u003e\u003cbr\u003eNOLC1 interacts with RNA polymerase I transcription machinery, fibrillarin, and nucleolin, coordinating rRNA transcription and processing. It also functions in nucleolar stress responses, where its relocalization signals cellular stress or DNA damage. By applying NOLC1 antibody, scientists can study how nucleolar function integrates with signaling pathways in cancer, aging, and neurodegeneration.\u003cbr\u003e\u003cbr\u003eNOLC1 mutations and expression changes contribute to oncogenesis. Overexpression correlates with tumor growth, while knockdown reduces proliferation. Because nucleolar size and activity are biomarkers of cancer, NOLC1 detection provides diagnostic and prognostic potential.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NOLC1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NOLC1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NOLC1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NOLC1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NOLC1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047304487277,"sku":"FY12841","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6d263238-e901-4c8c-ad0a-bf522fe43321.jpg?v=1782237047","url":"https:\/\/www.ebiohippo.com\/products\/nolc1-antibody-nucleolar-and-coiled-body-phosphoprotein-1-nopp140-bha17135743","provider":"BioHippo","version":"1.0","type":"link"}