{"product_id":"normal-human-peripheral-blood-mononuclear-cells-frozen-bhc18200286","title":"Normal Human Peripheral Blood Mononuclear Cells, Frozen","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table tr:nth-child(even) th{background:#dce8fb}\n.bhp-desc .bhp-spec-table tr:nth-child(even) td{background:#f0f6ff}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600;margin-bottom:10px}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444;margin:10px 0 14px}\n.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}\n.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}\n.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eHuman peripheral blood mononuclear cells (PBMCs) include all the cells present in the peripheral blood that have a round nucleus. It is thus a mix of lymphocytes, such as T, B and NK cells, and monocytes. Most cells present in PBMCs are lymphocytes, with T cells being the main cell type. The proportion of each cell type present can be determined by using cell specific marker combinations, for instance NK cells are characterized as CD3\u003csup\u003e-\u003c\/sup\u003eCD56\u003csup\u003e+\u003c\/sup\u003e cells. The use of these markers also allows for isolation of cell populations of interest. PBMCs are a critical and widely used cell source in the immunology, infectious disease, vaccine, cancer therapy and transplant immunology fields.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eImmune Cell Markers\u003c\/span\u003e\n\u003cp\u003eCryopreserved vial (100 x 10\u003csup\u003e6\u003c\/sup\u003e, 30 x 10\u003csup\u003e6\u003c\/sup\u003e, 10 x 10\u003csup\u003e6\u003c\/sup\u003e or 2 x 10\u003csup\u003e6\u003c\/sup\u003e cells) of freshly isolated primary human peripheral blood mononuclear cells (PBMCs) from a healthy donor, isolated from leukapheresis \/apheresis samples using Ficoll gradient. After isolation, the PBMCs were stained to identify sub populations and evaluated for viability by flow cytometry. Cells were cryopreserved in serum-free Cryostor CS10 at a controlled rate.\u003c\/p\u003e\n\u003ch2\u003eProduct Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eHarmonized Tariff Code\u003c\/th\u003e\n\u003ctd\u003e3002-5900\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cp\u003ePrepare a 50 ml conical tube with 10 ml of pre-warmed medium (10% FBS in IMDM or LGM). Quickly thaw cells in a 37°C water bath with constant and slow agitation. It is important to work quickly in the following steps to ensure high cell viability and recovery. Clean the outside of the vial with 70% ethanol and immediately transfer the entire content of the tube into the medium. Gently swirl the tube and centrifuge the cell suspension at 300 x g for 10 minutes at room temperature. Carefully remove the supernatant with a pipette without disturbing the pellet. Gently resuspend the cell pellet in 15-20 mL of warm medium. Centrifuge the cell suspension at 300 x g for 10 minutes at room temperature. Carefully remove the supernatant with a pipette without disturbing the pellet. Gently resuspend the cell pellet in warm medium and mix by gently flicking the tube. NOTE: Up to 30% of cell loss can be expected during washing steps. Cells are now ready for use in downstream applications.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cp style=\"margin:0 0 4px\"\u003e⚠ Avoid freeze\/thaw cycles.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003eRegulatory Information\u003c\/h2\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eLicense Required\u003c\/strong\u003eNo\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eLiving Modified Organism\u003c\/strong\u003eNo\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2\u003eLicense Disclosure\u003c\/h2\u003e\n\u003cdiv class=\"bhp-license-box\"\u003eTroubleshooting Guide: Visit bpsbioscience.com\/cell-line-faq for detailed troubleshooting instructions. For further questions, please email support@bpsbioscience.com.\u003c\/div\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eRelated Products: Cat. #79752, 79753\u003c\/p\u003e\n\u003c\/div\u003e","brand":"BPS Bioscience","offers":[{"title":"2 million cells","offer_id":53322323067245,"sku":"79059-4","price":50.0,"currency_code":"USD","in_stock":true},{"title":"10 million cells","offer_id":53322334863725,"sku":"79059-3","price":100.0,"currency_code":"USD","in_stock":true},{"title":"30 million cells","offer_id":53322334896493,"sku":"79059-1","price":220.0,"currency_code":"USD","in_stock":true},{"title":"100 million cells","offer_id":53322334929261,"sku":"79059-2","price":580.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/79059_1.png?v=1782155491","url":"https:\/\/www.ebiohippo.com\/products\/normal-human-peripheral-blood-mononuclear-cells-frozen-bhc18200286","provider":"BioHippo","version":"1.0","type":"link"}