{"product_id":"normal-human-primary-hepatic-stellate-cells-bhc10901958","title":"Normal Human Primary Hepatic Stellate Cells","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNormal Human Primary Hepatic Stellate Cells are human frozen primary cells from liver used for liver fibrosis, extracellular matrix remodeling, and hepatic stromal signaling studies. These cells display adherent, polygonal growth characteristics.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological source:\u003c\/strong\u003e Human (H. sapiens)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue origin:\u003c\/strong\u003e Liver\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Frozen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding guidance:\u003c\/strong\u003e 4,000 cells\/cm²\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e II\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCulture context:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eHepatic stellate cells regulate extracellular matrix turnover and are widely studied in liver injury and fibrosis models because of their ability to transition toward activated, matrix-producing states.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary stellate cells are used to examine activation-state transitions and fibrosis-associated matrix programs.\u003c\/li\u003e\n\u003cli\u003eCo-culture systems with hepatocytes, endothelial cells, or immune cells help model multicellular liver injury responses.\u003c\/li\u003e\n\u003cli\u003eResearchers frequently compare quiescent and activated phenotypes under defined cytokine or matrix conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAssess activation markers, matrix production, and profibrotic signaling under defined stimuli.\u003c\/li\u003e\n\u003cli\u003eModel hepatic stromal crosstalk in co-culture or conditioned-media experiments.\u003c\/li\u003e\n\u003cli\u003eUse in fibrosis-focused pathway studies and screening assays.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eProduct-specific data supplied for this listing\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Sinusoidal Endothelial Cell Growth Medium (TM086) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: for best results, allow cells to recover during the first 12 hours post-thaw. Change media 24 hours after thawing cells and every other day, thereafter.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWarranty:\u003c\/strong\u003e abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period”.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDisclaimer:\u003c\/strong\u003e 1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual\/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 4,000\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.\u003c\/li\u003e\n\u003cli\u003eAttachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.\u003c\/li\u003e\n\u003cli\u003eCryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC10901958\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"5x10\u003csup\u003e5\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53195757519213,"sku":"T5546","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/fyBvtO7DikEBy4eBPVIUAxJjWHX2VUDs7WWJDb6Z.png?v=1775322488","url":"https:\/\/www.ebiohippo.com\/products\/normal-human-primary-hepatic-stellate-cells-bhc10901958","provider":"BioHippo","version":"1.0","type":"link"}