{"product_id":"normal-human-primary-intestinal-epithelial-cells-transverse-colon-bhc10901944","title":"Normal Human Primary Intestinal Epithelial Cells Transverse Colon","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNormal Human Primary Intestinal Epithelial Cells Transverse Colon are human frozen primary cells from colon used for barrier biology, transport, host-pathogen interactions, and tissue-specific epithelial response studies. These cells display adherent, epithelial growth characteristics.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological source:\u003c\/strong\u003e Human (H. sapiens)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue origin:\u003c\/strong\u003e Colon\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Frozen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e II\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCulture context:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) . Intestinal Epithelial Cell Medium (TM090) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eEpithelial cells form polarized barriers that regulate transport, secretion, and defense at tissue interfaces. Their phenotype is shaped by cell polarity, junctional organization, and tissue-specific differentiation cues.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary epithelial models are widely used to examine barrier integrity, cytokine responses, and transport processes under physiologic conditions.\u003c\/li\u003e\n\u003cli\u003eResearchers often combine epithelial cells with stromal or immune components to model tissue microenvironments more faithfully.\u003c\/li\u003e\n\u003cli\u003eDonor-matched or disease-context epithelial cells help capture inter-individual heterogeneity in response assays.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eMeasure barrier integrity, junctional organization, and polarized responses in vitro.\u003c\/li\u003e\n\u003cli\u003eEvaluate cytokine, pathogen, or drug responses in tissue-relevant epithelial cultures.\u003c\/li\u003e\n\u003cli\u003eUse in co-culture or air-liquid interface-adjacent workflows when epithelial context matters.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eProduct-specific data supplied for this listing\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422) . Intestinal Epithelial Cell Medium (TM090) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWarranty:\u003c\/strong\u003e abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period”.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDisclaimer:\u003c\/strong\u003e 1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual\/institution for each order. If you have any questions regarding this, please contact us at orders@biohippo.com.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.\u003c\/li\u003e\n\u003cli\u003eAttachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.\u003c\/li\u003e\n\u003cli\u003eCryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC10901944\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"5x10\u003csup\u003e5\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53195756503405,"sku":"T5524","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/cNyxleWKqRxiMP8iyQRSHSJ5L1j9AzOiWyCIIw3c.png?v=1775322487","url":"https:\/\/www.ebiohippo.com\/products\/normal-human-primary-intestinal-epithelial-cells-transverse-colon-bhc10901944","provider":"BioHippo","version":"1.0","type":"link"}