{"product_id":"normal-human-primary-kidney-proximal-tubule-cells-bhc10901941","title":"Normal Human Primary Kidney Proximal Tubule Cells","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePrimary Normal Human Kidney Proximal Tubule (NhKPT) cells are isolated by positive magnetic selection from a heterogeneous population of cells taken from the cortical part of the kidney after cortex digestion. NhKPT cells are an integral part of the nephron. The product is supplied as frozen cells.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological source:\u003c\/strong\u003e Human (H. sapiens)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue origin:\u003c\/strong\u003e Kidney\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial-like\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Frozen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDonor history:\u003c\/strong\u003e Cortex\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e II\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCulture context:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003ePrimary cells provide a biologically relevant starting point for in vitro studies because they retain tissue-linked morphology, growth behavior, and donor-associated characteristics that are often reduced in immortalized systems.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary-cell models continue to be used when donor context, tissue specificity, and physiologic responsiveness are important.\u003c\/li\u003e\n\u003cli\u003eResearchers often combine primary cells with co-culture, matrix, or conditioned-media approaches to better reflect native microenvironments.\u003c\/li\u003e\n\u003cli\u003ePhenotypic characterization and careful tracking of culture conditions remain important for reproducible interpretation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eUse in tissue-relevant in vitro assays where morphology and donor context matter.\u003c\/li\u003e\n\u003cli\u003eMeasure phenotype, marker expression, or response to defined perturbations over time.\u003c\/li\u003e\n\u003cli\u003eIncorporate into co-culture or screening workflows requiring primary-cell context.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eProduct-specific data supplied for this listing\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM5520) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.Note: cells are terminally differentiated and can be propagated for at least 15 population doublings.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWarranty:\u003c\/strong\u003e abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period”.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.\u003c\/li\u003e\n\u003cli\u003eAttachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.\u003c\/li\u003e\n\u003cli\u003eCryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC10901941\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"5x10\u003csup\u003e5\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53195756667245,"sku":"T5520","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/8NiYw7PSsrrGuwpMOPtO48vmppmygclQGK8FVid1.png?v=1775322486","url":"https:\/\/www.ebiohippo.com\/products\/normal-human-primary-kidney-proximal-tubule-cells-bhc10901941","provider":"BioHippo","version":"1.0","type":"link"}