{"product_id":"novel-tau-biosensor-cell-line-p301sv2l-nk13-bhc10923067","title":"Novel Tau Biosensor Cell Line (P301SV2L (NK13))","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eT3488 (P301SV2L (NK13)) is one of two novel cell lines designed for the ultrasensitive detection of tau seeding activity, particularly in the context of Alzheimer’s disease research. These biosensor cell lines are engineered to express tau protein segments linked to fluorescent proteins, enabling fluorescence resonance energy transfer (FRET) based detection of tau aggregates.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Novel Tau Biosensor Cell Line (P301SV2L (NK13)) is supplied as an engineered cell line derived from Human kidney.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS(Regular*) + 1X GlutaMax + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. Cells are sensitive to post-thaw conditions. Change media 1 day post-thaw and allow cells to grow undistubed for 2-3 days.Allow cells to grow for at least 2-3 passages before using them for experimental assays. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eEach line offers unique features tailored to different research needs, enhancing both the sensitivity and specificity of tau pathology detection. Using a lentiviral vector, the tau protein segment (amino acids 246 to 378) with the P301S mutation was fused under a human cytomegalovirus (CMV) promoter to fluorescent proteins mCerulean3 or mClover3. The sequence linking the tau segment to the fluorescent proteins was optimized to improve FRET efficiency, essential for detecting tau aggregation dynamics.T3488 cells are designed for moderate expression of the tau fusion protein, making it ideal for applications where a balanced approach to sensitivity and cellular viability is needed. This cell line is particularly useful in studies where moderate to high concentrations of tau seeds are expected, providing reliable and linear dose-response measurements. Overall, this cell line is useful in screening potential therapeutics that aim to inhibit tau aggregation and supporting studies that track tau pathology over time to study neurodegenerative disease progression.Additional cell lines from this panel: Cat. T3489 - Novel Tau Biosensor Cell Line (P301SV2H (NK23))Other cell lines of interest: Cat. T3361 - TDP-43 (274-414) Stably Over-Expressing Biosensor Cell LineCat. T3362 - TDP-43 (262-414) Stably Over-Expressing Biosensor Cell Line Expression information reported for the model: tau protein segment (amino acids 246 to 378), mCerulean3, mClover3, P301SV2L.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS(Regular*) + 1X GlutaMax + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Note: Cells are sensitive to trypsin; Gentle Dissociation Solution (TM080) is recommended for subculture procedures. Cells are sensitive to post-thaw conditions. Change media 1 day post-thaw and allow cells to grow undistubed for 2-3 days.Allow cells to grow for at least 2-3 passages before using them for experimental assays. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 2,000,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180549628269,"sku":"T3488","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/3Nfp023CvWbhozJYsuJlStxnw6B0hOv680StuvS8.png?v=1774957879","url":"https:\/\/www.ebiohippo.com\/products\/novel-tau-biosensor-cell-line-p301sv2l-nk13-bhc10923067","provider":"BioHippo","version":"1.0","type":"link"}