{"product_id":"nprl2-antibody-nitrogen-permease-regulator-like-2-bha17135491","title":"NPRL2 Antibody \/ Nitrogen permease regulator-like 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNPRL2 Antibody \/ Nitrogen permease regulator-like 2 is a anti-NPRL2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NPRL2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNPRL2 antibody detects Nitrogen permease regulator-like 2, a component of the GATOR1 complex that inhibits mTORC1 signaling in response to amino acid deprivation. NPRL2 functions as a tumor suppressor and metabolic checkpoint regulator, linking nutrient sensing to cell growth and autophagy. The NPRL2 antibody is widely used in metabolism, oncology, and signal transduction research to study mTOR regulation, nutrient signaling, and tumor suppression.\u003cbr\u003e\u003cbr\u003eNPRL2 is encoded by the NPRL2 gene located on human chromosome 3p21.3, a region frequently deleted in several cancers. The protein is approximately 380 amino acids long and forms a complex with NPRL3 and DEPDC5 to constitute the GATOR1 inhibitory module. NPRL2 localizes to both cytoplasm and lysosomes, where it acts upstream of Rag GTPases to suppress mTORC1 activity under nutrient-limiting conditions.\u003cbr\u003e\u003cbr\u003eThe NPRL2 antibody detects a 45 kilodalton band by western blot and reveals punctate cytoplasmic staining consistent with lysosomal localization. NPRL2 is required for proper cellular adaptation to nutrient stress, promoting autophagy and metabolic reprogramming. Loss of NPRL2 results in constitutive mTORC1 activation, uncontrolled cell proliferation, and impaired stress response.\u003cbr\u003e\u003cbr\u003eAs a tumor suppressor, NPRL2 modulates DNA damage repair and enhances sensitivity to chemotherapeutic agents such as cisplatin. Reduced NPRL2 expression has been documented in lung, colorectal, and breast cancers, correlating with poor prognosis and therapy resistance. Restoring NPRL2 expression reactivates nutrient stress signaling and suppresses tumor growth through mTOR inhibition and apoptosis induction.\u003cbr\u003e\u003cbr\u003eThrough its dual role in nutrient sensing and cancer suppression, NPRL2 serves as a key regulator of cellular homeostasis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NPRL2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NPRL2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NPRL2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NPRL2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NPRL2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047293477229,"sku":"FY12588","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a6dfca30-52cc-46f6-ac72-e30a9d1847d8.jpg?v=1782237021","url":"https:\/\/www.ebiohippo.com\/products\/nprl2-antibody-nitrogen-permease-regulator-like-2-bha17135491","provider":"BioHippo","version":"1.0","type":"link"}