{"product_id":"npw-antibody-neuropeptide-w-bha17135955","title":"NPW Antibody \/ Neuropeptide W","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNPW Antibody \/ Neuropeptide W is a anti-NPW Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NPW\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNPW antibody detects Neuropeptide W, a bioactive peptide involved in energy homeostasis, stress response, and neuroendocrine regulation. The UniProt recommended name is Neuropeptide W (NPW). This neuropeptide exerts its physiological effects by binding to G protein-coupled receptors NPBWR1 and NPBWR2, activating downstream intracellular signaling cascades that regulate feeding, hormone secretion, and cardiovascular activity.\u003cbr\u003e\u003cbr\u003eFunctionally, NPW antibody identifies a 69-amino-acid precursor protein that is processed into two active peptides, NPW-23 and NPW-30, differing by their C-terminal truncation. These peptides are expressed in specific regions of the hypothalamus, brainstem, and peripheral tissues such as adrenal gland and stomach. NPW acts as an endogenous ligand for NPBWR1\/2, coupling primarily to Galphai\/o proteins and modulating cyclic AMP levels and calcium flux.\u003cbr\u003e\u003cbr\u003eThe NPW gene is located on chromosome 17p13.1 and encodes a prepropeptide that undergoes proteolytic processing and amidation to generate mature NPW peptides. In the central nervous system, NPW contributes to the regulation of feeding behavior, energy expenditure, and stress hormone release. It acts synergistically with leptin and insulin pathways to balance energy intake and expenditure. NPW also modulates arousal, locomotion, and nociceptive processing through brainstem circuits.\u003cbr\u003e\u003cbr\u003eIn peripheral tissues, NPW influences cardiovascular tone, adrenal steroidogenesis, and glucose metabolism. Its expression is regulated by nutritional state and stress exposure, reflecting its role in maintaining physiological equilibrium. In metabolic research, NPW is recognized as a peptide integrating hypothalamic signaling with peripheral energy regulation. Dysregulated NPW expression has been associated with obesity, stress-related disorders, and endocrine dysfunction.\u003cbr\u003e\u003cbr\u003eNPW antibody is widely used in neuroendocrinology, metabolism, and behavioral neuroscience research. It is suitable for immunohistochemistry, ELISA, and western blotting to detect NPW peptide distribution in brain and peripheral tissues. This antibody supports studies of neuropeptide signaling, hypothalamic regulation, and energy balance. In translational research, NPW serves as a potential biomarker for metabolic and stress-related conditions.\u003cbr\u003e\u003cbr\u003eStructurally, NPW peptides adopt flexible conformations stabilized by amidation and hydrophobic residues, allowing receptor binding with high affinity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NPW - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NPW - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NPW - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NPW - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NPW+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047310352749,"sku":"FY13053","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_bad5b289-3bc2-4464-8613-0462864b1c97.jpg?v=1782237078","url":"https:\/\/www.ebiohippo.com\/products\/npw-antibody-neuropeptide-w-bha17135955","provider":"BioHippo","version":"1.0","type":"link"}