{"product_id":"nqo1-antibody-bha17136355","title":"NQO1 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNQO1 Antibody is a anti-NQO1 Rabbit antibody Polyclonal (rabbit origin) supplied in Purified format. Recommended for workflows such as Western blot (WB), Immunofluorescence (IF), IHC-P, Flow cytometry (FACS) with listed reactivity in Human. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NQO1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IF, IHC-P, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNAD(P)H:quinone oxidoreductase 1 (NQO1) is a cytosolic flavoprotein that plays a central role in cellular defense against oxidative stress. As a 2-electron reductase, NQO1 catalyzes the reduction of quinones to hydroquinones, thereby preventing the formation of reactive oxygen species through redox cycling. This detoxifying activity contributes to the maintenance of redox homeostasis and protection of cells from oxidative damage. Research with an NQO1 antibody has provided valuable insights into its functions in normal physiology and disease.    \u003cbr\u003e\u003cbr\u003e  NQO1 expression is regulated by the Nrf2 pathway and is upregulated under stress conditions as part of the antioxidant response. Elevated levels of this enzyme have been reported in various cancers, suggesting a dual role in both cytoprotection and tumor progression. Studies using an NQO1 antibody have linked the protein to drug metabolism, chemoresistance, and its potential as a therapeutic target. Beyond oncology, NQO1 has been studied in relation to cardiovascular disease, neurodegeneration, and aging.    \u003cbr\u003e\u003cbr\u003e  The enzyme also stabilizes certain proteins by protecting them from proteasomal degradation, further highlighting its regulatory importance in cell survival pathways. Given these diverse roles, the use of an NQO1 antibody is valuable for detecting protein expression across tissues, evaluating stress responses, and exploring disease-specific alterations.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NQO1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NQO1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NQO1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NQO1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NQO1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047326703981,"sku":"RQ8937","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_c167da9e-92de-4ecd-b48b-90f14b180ba1.jpg?v=1772019450","url":"https:\/\/www.ebiohippo.com\/products\/nqo1-antibody-bha17136355","provider":"BioHippo","version":"1.0","type":"link"}