{"product_id":"nr1d1-antibody-nuclear-receptor-subfamily-1-group-d-member-1-bha17135870","title":"NR1D1 Antibody \/ Nuclear receptor subfamily 1 group D member 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNR1D1 Antibody \/ Nuclear receptor subfamily 1 group D member 1 is a anti-NR1D1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NR1D1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNR1D1 antibody detects Nuclear receptor subfamily 1 group D member 1, a transcriptional repressor that plays an essential role in circadian rhythm regulation, lipid metabolism, and inflammation. The UniProt recommended name is Nuclear receptor subfamily 1 group D member 1 (NR1D1), also known as Rev-erb alpha. NR1D1 is a ligand-dependent nuclear receptor that acts as a heme-binding transcriptional repressor, linking metabolic cues to circadian clock gene expression.\u003cbr\u003e\u003cbr\u003eFunctionally, NR1D1 antibody recognizes a 65 kDa nuclear protein that represses transcription by binding to Rev-erb response elements (ROREs) within target gene promoters. NR1D1 lacks the classical activation domain found in many nuclear receptors and instead recruits corepressor complexes such as NCoR and HDAC3 to silence transcription. Its oscillatory expression pattern forms part of the molecular circadian clock, antagonizing the activity of the transcriptional activator ROR alpha to maintain rhythmic gene expression. NR1D1 also regulates genes involved in lipid and glucose metabolism, mitochondrial biogenesis, and immune function.\u003cbr\u003e\u003cbr\u003eThe NR1D1 gene is located on chromosome 17q21.2 and encodes a protein composed of a DNA-binding domain with two zinc fingers and a ligand-binding domain that interacts with heme. Binding of heme modulates NR1D1 conformation and repressor activity, making it a redox-sensitive regulator of transcription. Through this mechanism, NR1D1 couples cellular metabolic state to circadian oscillations and energy balance. It directly represses key metabolic genes such as PGC-1alpha and BMAL1, thereby coordinating mitochondrial output and lipid storage cycles.\u003cbr\u003e\u003cbr\u003eIn metabolic tissues such as liver, skeletal muscle, and adipose tissue, NR1D1 acts as a gatekeeper for energy utilization. Its activation suppresses lipogenesis and promotes fatty acid oxidation. Dysregulation of NR1D1 expression has been associated with obesity, type 2 diabetes, and inflammatory disorders. Additionally, NR1D1 influences macrophage polarization and cytokine production, linking circadian control to immune homeostasis.\u003cbr\u003e\u003cbr\u003eNR1D1 antibody is widely used in chronobiology, endocrinology, and transcriptional regulation research. It is valuable for immunoblotting, chromatin immunoprecipitation (ChIP), and immunofluorescence to study nuclear localization, DNA binding, and protein interactions. The antibody helps elucidate NR1D1's involvement in circadian rhythm synchronization, metabolic adaptation, and disease-related transcriptional control. In cancer research, altered NR1D1 expression influences tumor metabolism and proliferation, reflecting its broader impact on cellular energy networks.\u003cbr\u003e\u003cbr\u003eStructurally, NR1D1 forms homodimers or heterodimers with nuclear receptor corepressors and interacts with histone deacetylases to remodel chromatin. Post-translational regulation includes phosphorylation and SUMOylation, which modulate its stability and DNA-binding affinity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NR1D1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NR1D1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NR1D1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NR1D1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NR1D1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047307731309,"sku":"FY12968","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2ec0196b-5ab1-4091-b3d1-bdf0e04fc818.jpg?v=1782237086","url":"https:\/\/www.ebiohippo.com\/products\/nr1d1-antibody-nuclear-receptor-subfamily-1-group-d-member-1-bha17135870","provider":"BioHippo","version":"1.0","type":"link"}