{"product_id":"nr1d2-antibody-rev-erb-beta-nuclear-receptor-subfamily-1-group-d-member-2-bha17136181","title":"NR1D2 Antibody \/ Rev-erb beta \/ Nuclear receptor subfamily 1 group D member 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNR1D2 Antibody \/ Rev-erb beta \/ Nuclear receptor subfamily 1 group D member 2 is a anti-NR1D2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NR1D2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNR1D2 antibody detects Nuclear receptor subfamily 1 group D member 2, a transcriptional repressor involved in circadian rhythm regulation, lipid metabolism, and inflammatory control. The UniProt recommended name is Nuclear receptor subfamily 1 group D member 2 (NR1D2). Also known as Rev-erb beta, this orphan nuclear receptor functions as a ligand-sensitive transcription factor that integrates metabolic and circadian signaling in peripheral tissues and the central clock.\u003cbr\u003e\u003cbr\u003eFunctionally, NR1D2 antibody identifies a 579-amino-acid protein that binds to Rev-erb response elements (RREs) in the promoters of target genes to repress transcription. NR1D2 interacts with the nuclear receptor co-repressor complex (NCoR\/HDAC3), recruiting histone deacetylases to chromatin and silencing gene expression. It regulates genes involved in lipid synthesis, gluconeogenesis, mitochondrial function, and the circadian machinery, particularly in coordination with its paralog NR1D1 (Rev-erb alpha).\u003cbr\u003e\u003cbr\u003eThe NR1D2 gene is located on chromosome 3p24.2 and is expressed rhythmically in liver, muscle, adipose tissue, and brain. Expression oscillates in antiphase to the transcriptional activators BMAL1 and CLOCK, ensuring temporal control of metabolic and circadian genes. NR1D2 also responds to heme as a natural ligand, linking metabolic redox state to transcriptional regulation.\u003cbr\u003e\u003cbr\u003ePathologically, altered NR1D2 function contributes to circadian rhythm disorders, metabolic syndrome, and inflammatory diseases. Reduced activity disrupts daily metabolic cycles and leads to increased lipid accumulation and insulin resistance. Conversely, overactivation suppresses inflammatory gene expression and may offer therapeutic benefits in metabolic inflammation. Research using NR1D2 antibody supports studies in chronobiology, metabolism, and transcriptional regulation.\u003cbr\u003e\u003cbr\u003eNR1D2 antibody is validated for western blotting, immunofluorescence, and chromatin immunoprecipitation to detect nuclear transcription factors. \u003cbr\u003e\u003cbr\u003eStructurally, Nuclear receptor subfamily 1 group D member 2 contains a conserved DNA-binding domain with two zinc fingers, a ligand-binding domain for heme interaction, and a repressor interface for co-repressor complex recruitment. Its activity oscillates with diurnal rhythm, influencing both chromatin accessibility and transcriptional output. This antibody enables exploration of NR1D2's role in circadian transcriptional repression and metabolic synchronization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NR1D2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NR1D2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NR1D2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NR1D2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NR1D2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047320609133,"sku":"FY13279","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2bcb1418-ebc7-46ce-87f8-3399a644bb88.jpg?v=1782237099","url":"https:\/\/www.ebiohippo.com\/products\/nr1d2-antibody-rev-erb-beta-nuclear-receptor-subfamily-1-group-d-member-2-bha17136181","provider":"BioHippo","version":"1.0","type":"link"}