{"product_id":"nr1h3-antibody-oxysterols-receptor-lxr-alpha-bha17136058","title":"NR1H3 Antibody \/ Oxysterols receptor LXR-alpha","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNR1H3 Antibody \/ Oxysterols receptor LXR-alpha is a anti-NR1H3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NR1H3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNR1H3 antibody detects Oxysterols receptor LXR-alpha, a nuclear receptor that regulates lipid metabolism, inflammation, and cholesterol homeostasis. The UniProt recommended name is Oxysterols receptor LXR-alpha (NR1H3). This transcription factor belongs to the nuclear receptor superfamily and functions as a ligand-activated regulator controlling expression of genes involved in lipid transport and metabolism.\u003cbr\u003e\u003cbr\u003eFunctionally, NR1H3 antibody identifies a 447-amino-acid receptor that binds oxysterol ligands, including 24(S)-hydroxycholesterol and 27-hydroxycholesterol. Upon activation, NR1H3 forms heterodimers with RXR and binds to LXR response elements in target gene promoters, stimulating transcription of ABCA1, SREBP-1c, and APOE to promote cholesterol efflux and fatty acid synthesis.\u003cbr\u003e\u003cbr\u003eThe NR1H3 gene is located on chromosome 11p11.2 and is highly expressed in liver, macrophages, and intestine. Its activity coordinates lipid handling in hepatocytes and immune cells, maintaining systemic cholesterol balance and preventing lipid accumulation. NR1H3 also modulates inflammatory gene expression by antagonizing NF-kappaB signaling.\u003cbr\u003e\u003cbr\u003ePathologically, dysregulation of NR1H3 contributes to atherosclerosis, fatty liver disease, and metabolic syndrome. Genetic variants in NR1H3 have been associated with autoimmune and neuroinflammatory disorders, including multiple sclerosis. Research using NR1H3 antibody supports studies in lipid regulation, transcriptional control, and metabolic disease mechanisms.\u003cbr\u003e\u003cbr\u003eNR1H3 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect nuclear receptors and transcription factors. \u003cbr\u003e\u003cbr\u003eStructurally, Oxysterols receptor LXR-alpha contains a DNA-binding zinc finger domain and a ligand-binding domain that mediates dimerization and transcriptional activation. This antibody supports analysis of NR1H3's regulatory functions in cholesterol and lipid homeostasis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NR1H3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NR1H3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NR1H3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NR1H3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NR1H3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047314055533,"sku":"FY13156","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a6951f8a-1c75-4e3c-9b1b-181d3270f049.jpg?v=1782237113","url":"https:\/\/www.ebiohippo.com\/products\/nr1h3-antibody-oxysterols-receptor-lxr-alpha-bha17136058","provider":"BioHippo","version":"1.0","type":"link"}