{"product_id":"nr3c2-reporter-lentivirus-bhv19400219","title":"NR3C2 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eNR3C2 encodes the mineralocorticoid receptor (MR), a ligand-activated nuclear receptor that mediates the effects of the hormone aldosterone. Upon ligand binding, MR translocates to the nucleus and regulates target genes through hormone response elements. MR is central to water and electrolyte balance, blood pressure regulation, and the control of inflammation and fibrosis in the kidney and cardiovascular system. Although MR shares response elements with the glucocorticoid receptor, it drives MR-specific outputs such as upregulation of EGFR. Progesterone and estrogen normally act as MR antagonists, but certain MR mutations convert progesterone into an agonist, causing early-onset hypertension that can worsen in pregnancy. MR dysregulation is broadly linked to hypertension.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe NR3C2 Reporter Lentivirus is a transcription-factor reporter system for robust detection of mineralocorticoid receptor transcriptional activity in mammalian cells. The construct uses tandem repeats of the NR3C2 response element derived from the EGFR promoter to specifically monitor MR activity, coupled to a minimal promoter driving a fluorescent or luminescent reporter. A constitutively expressed selection marker and optional secondary reporter support stable polyclonal reporter cell lines with readout by microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding transient-transfection variability. Particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, supporting research on aldosterone signaling, blood pressure regulation, and hypertension.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the NR3C2 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251622895981,"sku":"LTV-0110-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53362031755629,"sku":"LTV-0110-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53362031788397,"sku":"LTV-0110-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53362031821165,"sku":"LTV-0110-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53362031853933,"sku":"LTV-0110-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53362031886701,"sku":"LTV-0110-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/NR3C2-TAG-Puro-Thumb.jpg?v=1776934148","url":"https:\/\/www.ebiohippo.com\/products\/nr3c2-reporter-lentivirus-bhv19400219","provider":"BioHippo","version":"1.0","type":"link"}