{"product_id":"nrcam-antibody-neuronal-cell-adhesion-molecule-bha17135440","title":"NRCAM Antibody \/ Neuronal cell adhesion molecule","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNRCAM Antibody \/ Neuronal cell adhesion molecule is a anti-NRCAM Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NRCAM\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNRCAM antibody detects Neuronal cell adhesion molecule, a type I transmembrane glycoprotein belonging to the L1 family of cell adhesion molecules (CAMs). NRCAM promotes axonal guidance, synapse formation, and cell-cell communication during nervous system development. The NRCAM antibody is widely used in neuroscience, developmental biology, and neuro-oncology research to study neuronal adhesion, axonal fasciculation, and signal transduction.\u003cbr\u003e\u003cbr\u003eNRCAM is encoded by the NRCAM gene located on human chromosome 7q31.1. The protein is approximately 135 kilodaltons and comprises six immunoglobulin-like domains, five fibronectin type III repeats, a single transmembrane segment, and a short cytoplasmic tail containing ankyrin-binding motifs. These structural features allow NRCAM to interact with both extracellular ligands and intracellular cytoskeletal adaptors, linking adhesion to signal transduction and cytoskeletal organization.\u003cbr\u003e\u003cbr\u003eThe NRCAM antibody detects a 135 kilodalton band by western blot and exhibits strong membrane and axonal staining in neuronal cultures and brain tissue. NRCAM mediates homophilic and heterophilic interactions with other cell adhesion molecules, including contactin and L1CAM, forming complexes that regulate neurite outgrowth, axonal pathfinding, and synapse stabilization. In developing neurons, NRCAM clusters at growth cones, coordinating adhesion with actin dynamics and microtubule extension.\u003cbr\u003e\u003cbr\u003eNRCAM also participates in signaling cascades involving the MAPK and PI3K pathways, modulating neuronal survival and differentiation. In non-neuronal tissues, NRCAM contributes to cell migration and epithelial morphogenesis, while in cancer, its overexpression promotes tumor cell adhesion and metastasis, particularly in melanoma, glioma, and prostate carcinoma.\u003cbr\u003e\u003cbr\u003eGenetic and expression studies link NRCAM variants to neurodevelopmental and psychiatric conditions, including autism spectrum disorder and schizophrenia, suggesting that altered cell adhesion signaling may underlie synaptic dysfunction.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NRCAM - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NRCAM - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NRCAM - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NRCAM - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NRCAM+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047291806061,"sku":"FY12537","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7a77fec0-5b30-45de-8ff6-9c7945f1a70d.jpg?v=1782237021","url":"https:\/\/www.ebiohippo.com\/products\/nrcam-antibody-neuronal-cell-adhesion-molecule-bha17135440","provider":"BioHippo","version":"1.0","type":"link"}