{"product_id":"nrf2-antibody-nuclear-factor-erythroid-2-related-factor-2-nfe2l2-bha17136071","title":"NRF2 Antibody \/ Nuclear factor erythroid 2-related factor 2 \/ NFE2L2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNRF2 Antibody \/ Nuclear factor erythroid 2-related factor 2 \/ NFE2L2 is a anti-NRF2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NRF2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNRF2 antibody detects Nuclear factor erythroid 2-related factor 2, a transcription factor that regulates antioxidant defense and cellular detoxification. The UniProt recommended name is Nuclear factor erythroid 2-related factor 2 (NFE2L2). This basic leucine zipper (bZIP) transcription factor activates expression of antioxidant response element (ARE)-driven genes that protect cells against oxidative and electrophilic stress.\u003cbr\u003e\u003cbr\u003eFunctionally, NRF2 antibody identifies a 605-amino-acid protein that binds to DNA as a heterodimer with small Maf proteins. Under basal conditions, NRF2 is bound by KEAP1 in the cytoplasm and targeted for proteasomal degradation. Upon oxidative stress, NRF2 escapes KEAP1 repression, translocates to the nucleus, and induces genes encoding detoxification enzymes, antioxidant proteins, and drug-metabolizing enzymes such as NQO1, HO-1, and GCLM.\u003cbr\u003e\u003cbr\u003eThe NFE2L2 gene is located on chromosome 2q31.2 and is expressed in multiple tissues, with high levels in liver, lung, and kidney. NRF2 acts as a master regulator of cellular defense mechanisms, maintaining redox balance, xenobiotic metabolism, and mitochondrial function.\u003cbr\u003e\u003cbr\u003ePathologically, persistent NRF2 activation contributes to tumorigenesis and drug resistance, while impaired NRF2 signaling increases susceptibility to oxidative injury and chronic diseases such as neurodegeneration and fibrosis. Research using NRF2 antibody supports studies in transcriptional regulation, redox signaling, and cytoprotection.\u003cbr\u003e\u003cbr\u003eNRF2 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect transcription factors involved in antioxidant defense. \u003cbr\u003e\u003cbr\u003eStructurally, Nuclear factor erythroid 2-related factor 2 contains multiple Neh (Nrf2-ECH homology) domains that mediate DNA binding, KEAP1 interaction, and transcriptional activation. This antibody aids investigation of NRF2's dynamic regulation and its role in cellular adaptation to stress.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NRF2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NRF2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NRF2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NRF2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NRF2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047314219373,"sku":"FY13169","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_92a627b5-a829-4235-a148-8abaf879356a.jpg?v=1782237093","url":"https:\/\/www.ebiohippo.com\/products\/nrf2-antibody-nuclear-factor-erythroid-2-related-factor-2-nfe2l2-bha17136071","provider":"BioHippo","version":"1.0","type":"link"}