{"product_id":"nsdhl-antibody-nadp-dependent-steroid-dehydrogenase-like-protein-bha17135235","title":"NSDHL Antibody \/ NADP-dependent steroid dehydrogenase-like protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNSDHL Antibody \/ NADP-dependent steroid dehydrogenase-like protein is a anti-NSDHL Rabbit antibody Recombinant Rabbit Monoclonal clone 30N66 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NSDHL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30N66, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IP\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNSDHL antibody is designed to detect NAD(P)-dependent steroid dehydrogenase-like protein, an essential enzyme of the cholesterol biosynthetic pathway. NSDHL is localized to the endoplasmic reticulum membrane and functions as a C-4 sterol dehydrogenase, catalyzing the removal of methyl groups from sterol intermediates. This enzymatic activity is necessary for producing cholesterol and other sterols that are critical for membrane structure, signaling, and developmental processes.\u003cbr\u003e\u003cbr\u003eNSDHL antibody is widely used in studies of metabolism, developmental biology, and genetic disorders. Mutations in the NSDHL gene are associated with CHILD syndrome (congenital hemidysplasia with ichthyosiform erythroderma and limb defects), an X-linked dominant disorder characterized by skin abnormalities and limb malformations. Detecting NSDHL with specific antibodies enables researchers to evaluate expression and function in disease models and clinical studies.\u003cbr\u003e\u003cbr\u003eApplications of NSDHL antibody include western blotting, immunohistochemistry, and immunofluorescence. In western blot assays, NSDHL antibody detects the protein with high specificity, confirming its expression across cell and tissue samples. Immunohistochemistry reveals distribution patterns in tissues involved in sterol metabolism, while immunofluorescence highlights its endoplasmic reticulum localization in cultured cells. These experimental approaches provide important insights into cholesterol biosynthesis at the molecular level.\u003cbr\u003e\u003cbr\u003eNSDHL plays a vital role in cholesterol metabolism, and dysregulation of its activity has broader implications for human health. Abnormal sterol accumulation contributes to metabolic disease, cardiovascular dysfunction, and neurodevelopmental abnormalities. Using NSDHL antibody, researchers can track enzyme activity and explore how disruptions in cholesterol biosynthesis impact cellular and systemic function.\u003cbr\u003e\u003cbr\u003eBeyond inherited disorders, NSDHL has been implicated in cancer biology. Cholesterol metabolism supports rapid tumor growth, and enzymes in the biosynthetic pathway are increasingly recognized as potential therapeutic targets. Monitoring NSDHL expression with antibodies allows investigators to connect sterol metabolism with oncogenic processes, highlighting new avenues for intervention.\u003cbr\u003e\u003cbr\u003eNSDHL antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NSDHL - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NSDHL - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NSDHL - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NSDHL - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NSDHL+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047285023085,"sku":"FY12332","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_9ccace21-3411-4576-8c12-97af18fe9b07.jpg?v=1772019288","url":"https:\/\/www.ebiohippo.com\/products\/nsdhl-antibody-nadp-dependent-steroid-dehydrogenase-like-protein-bha17135235","provider":"BioHippo","version":"1.0","type":"link"}