{"product_id":"nsdhl-antibody-nadp-dependent-steroid-dehydrogenase-like-protein-bha17135659","title":"NSDHL Antibody \/ NADP dependent steroid dehydrogenase like protein","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNSDHL Antibody \/ NADP dependent steroid dehydrogenase like protein is a anti-NSDHL Rabbit antibody Recombinant Rabbit Monoclonal clone 30N01 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NSDHL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30N01, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNSDHL antibody detects NADP dependent steroid dehydrogenase like protein, encoded by the NSDHL gene. This protein is also known as sterol 4 alpha carboxylate 3 dehydrogenase decarboxylating, H105E3, and the human homolog of the mouse bare patches gene. NSDHL is an essential enzyme in the cholesterol biosynthetic pathway. It functions in the endoplasmic reticulum, catalyzing oxidative decarboxylation of C4 methylated sterol intermediates. These steps are necessary for the removal of methyl groups during the conversion of lanosterol to cholesterol. Because cholesterol is critical for membranes, steroid hormones, and signaling lipids, NSDHL plays a central role in both basic metabolism and developmental biology.\u003cbr\u003e\u003cbr\u003eNSDHL antibody is widely applied in studies of cholesterol metabolism, embryogenesis, dermatology, and disease research. Mutations in NSDHL cause CHILD syndrome, a rare X linked disorder that results in congenital hemidysplasia, ichthyosiform erythroderma, and limb defects. By detecting NSDHL, researchers can trace how mutations impair cholesterol synthesis and lead to toxic sterol accumulation. The protein is expressed in multiple tissues with high metabolic activity, including liver, brain, and skin, making it a valuable marker for sterol pathway integrity.\u003cbr\u003e\u003cbr\u003eApplications of NSDHL antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blot assays identify NSDHL expression in tissue lysates, immunohistochemistry highlights spatial distribution in liver and epidermis, and immunofluorescence reveals its localization in the endoplasmic reticulum. These methods allow researchers to connect protein expression to sterol metabolism and developmental outcomes. Because cholesterol synthesis is fundamental across organisms, NSDHL antibody has relevance in both basic biology and translational studies.\u003cbr\u003e\u003cbr\u003eBiochemically, NSDHL functions alongside other C4 demethylation enzymes, including sterol C4 methyl oxidase and 3 beta hydroxysteroid dehydrogenase like proteins. Together they coordinate the removal of C4 methyl groups, ensuring accurate production of downstream sterols. Disruption of NSDHL activity interrupts this sequence, creating intermediates that impair membranes and signal transduction. By applying NSDHL antibody, scientists can evaluate enzyme function within the broader sterol demethylation complex.\u003cbr\u003e\u003cbr\u003eBeyond congenital syndromes, NSDHL is increasingly studied in oncology. Cancer cells reprogram metabolism to support proliferation, and cholesterol synthesis is frequently upregulated. NSDHL expression correlates with tumor growth and resistance to certain therapies. Inhibiting sterol biosynthetic enzymes, including NSDHL, is a promising therapeutic strategy under investigation. The antibody therefore has applications in cancer metabolism research, where monitoring NSDHL may reveal vulnerabilities to metabolic intervention.\u003cbr\u003e\u003cbr\u003eCholesterol synthesis also intersects with neurobiology. The brain relies heavily on de novo cholesterol production, and NSDHL mutations or altered expression affect myelination and neuronal function. By detecting NSDHL with antibody based assays, researchers can study sterol metabolism in neurodevelopmental disorders and neurodegeneration. These insights extend to understanding how cholesterol imbalances influence synaptic function and cognition.\u003cbr\u003e\u003cbr\u003eNSDHL antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NSDHL - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NSDHL - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NSDHL - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NSDHL - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NSDHL+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047298621805,"sku":"FY12756","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b9f5be93-0879-4bfa-af65-b05f089b1c00.jpg?v=1772019337","url":"https:\/\/www.ebiohippo.com\/products\/nsdhl-antibody-nadp-dependent-steroid-dehydrogenase-like-protein-bha17135659","provider":"BioHippo","version":"1.0","type":"link"}