{"product_id":"nsdhl-antibody-sterol-4-alpha-carboxylate-3-dehydrogenase-bha17136057","title":"NSDHL Antibody \/ Sterol-4-alpha-carboxylate 3-dehydrogenase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNSDHL Antibody \/ Sterol-4-alpha-carboxylate 3-dehydrogenase is a anti-NSDHL Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: ER.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NSDHL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNSDHL antibody detects Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating, a membrane-associated enzyme involved in cholesterol biosynthesis. The UniProt recommended name is Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (NSDHL). This enzyme catalyzes oxidative decarboxylation of sterol intermediates during the post-squalene steps of cholesterol production, playing a crucial role in lipid homeostasis and membrane integrity.\u003cbr\u003e\u003cbr\u003eFunctionally, NSDHL antibody identifies a 373-amino-acid enzyme localized to the endoplasmic reticulum membrane. NSDHL acts sequentially with other enzymes such as HSD17B7 and EBP to remove carboxyl groups from C4-methyl sterols, facilitating the conversion of lanosterol to cholesterol. Its catalytic activity contributes to maintaining sterol balance essential for cell signaling and membrane function.\u003cbr\u003e\u003cbr\u003eThe NSDHL gene is located on chromosome Xq28 and is highly expressed in liver, skin, and brain tissues. Its activity supports embryonic development, epidermal differentiation, and lipid raft formation in cell membranes. NSDHL is evolutionarily conserved and tightly regulated to ensure steady cholesterol output.\u003cbr\u003e\u003cbr\u003ePathologically, mutations in NSDHL cause CHILD syndrome (Congenital Hemidysplasia with Ichthyosiform erythroderma and Limb Defects), a rare X-linked disorder involving defective cholesterol metabolism and asymmetric skin and limb malformations. NSDHL dysfunction also disrupts sterol intermediates, leading to toxic accumulation of precursors. Research using NSDHL antibody supports studies in lipid metabolism, cholesterol biosynthesis, and genetic disorders of sterol processing.\u003cbr\u003e\u003cbr\u003eNSDHL antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect sterol biosynthetic enzymes. \u003cbr\u003e\u003cbr\u003eStructurally, Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating contains a short-chain dehydrogenase\/reductase catalytic domain and transmembrane segments that anchor it to the endoplasmic reticulum. This antibody enables study of NSDHL's role in sterol conversion and metabolic regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NSDHL - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NSDHL - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NSDHL - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NSDHL - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NSDHL+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047314252141,"sku":"FY13155","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_4fb81cfc-a55b-4a1a-b2e9-c0eee3c47f53.jpg?v=1782237118","url":"https:\/\/www.ebiohippo.com\/products\/nsdhl-antibody-sterol-4-alpha-carboxylate-3-dehydrogenase-bha17136057","provider":"BioHippo","version":"1.0","type":"link"}