{"product_id":"nsl1-antibody-kinetochore-associated-protein-nsl1-bha17135918","title":"NSL1 Antibody \/ Kinetochore-associated protein NSL1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNSL1 Antibody \/ Kinetochore-associated protein NSL1 is a anti-NSL1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NSL1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNSL1 antibody detects Kinetochore-associated protein NSL1, a core component of the MIS12 complex required for proper chromosome segregation during mitosis. The UniProt recommended name is Kinetochore-associated protein NSL1 (NSL1). This evolutionarily conserved protein plays a crucial role in the assembly and maintenance of kinetochore structure, ensuring accurate attachment of spindle microtubules to centromeric chromatin during cell division.\u003cbr\u003e\u003cbr\u003eFunctionally, NSL1 antibody identifies a 281-amino-acid nuclear and centromere-localized protein that forms part of the MIS12 complex, along with MIS12, DSN1, and PMF1. This complex acts as a central linker connecting the inner kinetochore, bound to centromeric DNA, with the outer kinetochore microtubule-binding components. NSL1 stabilizes kinetochore architecture and promotes tension-dependent microtubule attachment, which is essential for accurate chromosome alignment and segregation.\u003cbr\u003e\u003cbr\u003eThe NSL1 gene is located on chromosome 1q32.3 and encodes a coiled-coil domain-containing protein that contributes to kinetochore-spindle interactions. Through its association with the MIS12 complex, NSL1 participates in recruiting key outer kinetochore proteins such as NDC80 and KNL1, forming the KMN network that mediates microtubule attachment and spindle checkpoint signaling. Disruption of NSL1 function causes chromosome mis-segregation, aneuploidy, and mitotic arrest.\u003cbr\u003e\u003cbr\u003eBeyond its structural role, NSL1 coordinates with spindle assembly checkpoint proteins to monitor proper kinetochore-microtubule tension. By serving as a molecular bridge between centromeric chromatin and spindle fibers, NSL1 ensures mitotic fidelity and genomic stability. Dysregulation or mutation of kinetochore components, including NSL1, contributes to chromosomal instability observed in cancer cells, highlighting its importance in tumor biology.\u003cbr\u003e\u003cbr\u003eNSL1 antibody is widely used in cell cycle, mitosis, and chromosome segregation research. It is suitable for immunofluorescence, immunoblotting, and immunoprecipitation to study kinetochore organization and spindle checkpoint regulation. In cancer biology, this antibody supports investigations into chromosomal instability mechanisms, mitotic defects, and checkpoint adaptation. It also aids in mapping kinetochore composition across cell cycle phases.\u003cbr\u003e\u003cbr\u003eStructurally, NSL1 contains coiled-coil motifs and conserved interaction domains that facilitate complex assembly with MIS12 and DSN1. Its function depends on dynamic phosphorylation and protein-protein interactions during metaphase and anaphase transitions.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NSL1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NSL1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NSL1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NSL1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NSL1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047309730157,"sku":"FY13016","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_1ff084c8-6daf-4654-892c-76d7f7806a26.jpg?v=1782237074","url":"https:\/\/www.ebiohippo.com\/products\/nsl1-antibody-kinetochore-associated-protein-nsl1-bha17135918","provider":"BioHippo","version":"1.0","type":"link"}