{"product_id":"nsmce4a-antibody-non-smc-element-4-homolog-a-bha17135786","title":"NSMCE4A Antibody \/ Non-SMC element 4 homolog A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNSMCE4A Antibody \/ Non-SMC element 4 homolog A is a anti-NSMCE4A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NSMCE4A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNSMCE4A antibody detects Non-structural maintenance of chromosomes element 4A, a core component of the SMC5\/6 complex involved in chromosome maintenance, DNA repair, and replication fork stability. Encoded by the NSMCE4A gene on chromosome 10q25.3, this protein forms part of the essential SMC5\/6 complex that ensures proper segregation of sister chromatids and preserves genome integrity during cell division and stress. NSMCE4A serves as a bridging subunit, connecting the SMC5 and SMC6 heterodimer to regulatory and enzymatic cofactors that control DNA repair and replication restart.\u003cbr\u003e\u003cbr\u003eStructurally, NSMCE4A is a 34 kilodalton nuclear protein that interacts directly with SMC5 and SMC6 through its C-terminal coiled-coil domains, forming a stable hinge component of the complex. Its N-terminal region mediates associations with additional subunits such as NSMCE1 and NSMCE2, which confer ubiquitin ligase activity and DNA damage response signaling capacity. This modular organization enables NSMCE4A to coordinate complex assembly and spatial localization at damaged DNA sites.\u003cbr\u003e\u003cbr\u003eThe NSMCE4A antibody is widely used in molecular biology, DNA repair, and cell cycle research to study chromosomal maintenance, recombination, and replication dynamics. Western blot analysis identifies a 34 kilodalton band corresponding to NSMCE4A, while immunofluorescence shows punctate nuclear staining that increases following DNA damage. This antibody provides a powerful reagent for investigating the SMC5\/6 complex and its role in genome stability.\u003cbr\u003e\u003cbr\u003eFunctionally, NSMCE4A is required for homologous recombination repair, resolution of DNA replication stress, and suppression of chromosomal rearrangements. Loss of NSMCE4A disrupts complex assembly, leading to DNA double-strand break accumulation and mitotic abnormalities. The SMC5\/6 complex, including NSMCE4A, also regulates the maintenance of repetitive DNA sequences and prevents unscheduled recombination at telomeres and ribosomal DNA. Aberrant NSMCE4A expression or mutation has been implicated in genome instability disorders and cancer, where defective repair pathways promote genomic rearrangements. The NSMCE4A antibody enables sensitive detection and quantification of this repair factor in mechanistic studies of DNA integrity, replication stress, and chromosome segregation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NSMCE4A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NSMCE4A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NSMCE4A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NSMCE4A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NSMCE4A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047304847725,"sku":"FY12884","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_340648bd-ba63-4cb4-bc10-89700e369291.jpg?v=1782237054","url":"https:\/\/www.ebiohippo.com\/products\/nsmce4a-antibody-non-smc-element-4-homolog-a-bha17135786","provider":"BioHippo","version":"1.0","type":"link"}