{"product_id":"nsun7-antibody-nop2-sun-rna-methyltransferase-family-member-7-bha17135830","title":"NSUN7 Antibody \/ NOP2\/Sun RNA methyltransferase family member 7","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNSUN7 Antibody \/ NOP2\/Sun RNA methyltransferase family member 7 is a anti-NSUN7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NSUN7\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNSUN7 antibody detects NOP2\/Sun RNA methyltransferase family member 7, an enzyme responsible for methylating cytosine residues in RNA molecules, particularly within mitochondrial and spermatozoal transcripts. Encoded by the NSUN7 gene on chromosome 4q26, this enzyme belongs to the RNA cytosine methyltransferase family, which regulates RNA stability, translation, and processing through site-specific methylation. NSUN7 plays key roles in sperm motility, mitochondrial metabolism, and epigenetic control of gene expression.\u003cbr\u003e\u003cbr\u003eStructurally, NSUN7 is a 721-amino-acid mitochondrial and cytoplasmic protein of approximately 80 kilodaltons containing a conserved catalytic cysteine motif and an S-adenosylmethionine (SAM)-binding domain required for methyl group transfer. It is expressed predominantly in testes, brain, and metabolic tissues, reflecting its involvement in mitochondrial gene regulation and male fertility. The enzyme localizes to the mitochondrial matrix and cytoplasmic ribonucleoprotein complexes, where it modifies tRNAs and mRNAs.\u003cbr\u003e\u003cbr\u003eThe NSUN7 antibody is widely used in epigenetics, reproductive biology, and mitochondrial research to study RNA methylation, translational regulation, and sperm function. Western blot analysis detects an 80 kilodalton band corresponding to NSUN7, while immunofluorescence reveals cytoplasmic and mitochondrial staining in germ cells and metabolic tissues. This antibody supports studies examining RNA modification-dependent regulation of gene expression.\u003cbr\u003e\u003cbr\u003eFunctionally, NSUN7 contributes to mitochondrial energy homeostasis and sperm motility by methylating specific RNA molecules required for mitochondrial translation. Mutations in NSUN7 cause male infertility due to defective sperm motility and impaired mitochondrial activity. Emerging evidence suggests that RNA methylation by NSUN7 influences cellular energy balance and oxidative stress responses beyond the reproductive system. The NSUN7 antibody provides a powerful reagent for studying RNA modification pathways, mitochondrial RNA biology, and epigenetic regulation of metabolism.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NSUN7 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NSUN7 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NSUN7 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NSUN7 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NSUN7+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047306846573,"sku":"FY12928","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e5bab68d-c827-4179-aa0f-9594c61b4f3f.jpg?v=1782237060","url":"https:\/\/www.ebiohippo.com\/products\/nsun7-antibody-nop2-sun-rna-methyltransferase-family-member-7-bha17135830","provider":"BioHippo","version":"1.0","type":"link"}