{"product_id":"ntmt2-antibody-n-terminal-methyltransferase-2-bha17135787","title":"NTMT2 Antibody \/ N-terminal methyltransferase 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNTMT2 Antibody \/ N-terminal methyltransferase 2 is a anti-NTMT2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NTMT2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNTMT2 antibody detects Protein N-terminal methyltransferase 2, an enzyme responsible for alpha-N-terminal methylation of proteins involved in chromatin organization, DNA repair, and transcriptional regulation. Encoded by the NTMT2 gene on chromosome 21q21.3, this enzyme belongs to the class I S-adenosylmethionine (SAM)-dependent methyltransferase family and acts in conjunction with its homolog NTMT1 to modify the alpha-amino group of N-terminal residues. NTMT2-mediated methylation contributes to protein stability, localization, and interaction dynamics in the nucleus and cytoplasm.\u003cbr\u003e\u003cbr\u003eStructurally, NTMT2 is an approximately 32 kilodalton enzyme localized primarily to the nucleus, where it targets proteins containing an N-terminal X-Pro-Lys motif for mono-, di-, or trimethylation. It possesses a conserved methyltransferase catalytic domain that binds SAM as a methyl donor and a flexible substrate recognition pocket that determines sequence specificity. Through these enzymatic properties, NTMT2 regulates key nuclear processes, including chromatin compaction and DNA damage signaling.\u003cbr\u003e\u003cbr\u003eThe NTMT2 antibody is widely used in epigenetics, molecular biology, and proteomics research to study protein methylation, nuclear regulation, and post-translational modification networks. Western blot analysis identifies a 32 kilodalton band corresponding to NTMT2, while immunofluorescence reveals strong nuclear and nucleolar localization consistent with its methyltransferase function. This antibody provides a robust reagent for quantifying NTMT2 expression and exploring its role in gene regulation and chromatin state control.\u003cbr\u003e\u003cbr\u003eFunctionally, NTMT2 catalyzes the methylation of numerous substrates, including centromere-associated proteins and chromatin regulators, influencing their stability and recruitment to DNA-binding complexes. It works in coordination with NTMT1 to maintain proper levels of N-terminal methylation, essential for genomic stability and cell proliferation. Dysregulation of NTMT2 expression has been linked to cancer, where altered methylation affects DNA repair and transcriptional reprogramming. The NTMT2 antibody supports mechanistic studies of post-translational modification systems and their contribution to epigenetic control and cellular differentiation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NTMT2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NTMT2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NTMT2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NTMT2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NTMT2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047305208173,"sku":"FY12885","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a0a96b08-a9ce-4923-8eba-93318b0222b0.jpg?v=1782237052","url":"https:\/\/www.ebiohippo.com\/products\/ntmt2-antibody-n-terminal-methyltransferase-2-bha17135787","provider":"BioHippo","version":"1.0","type":"link"}