{"product_id":"ntn4-antibody-netrin-4-bha17136180","title":"NTN4 Antibody \/ Netrin 4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNTN4 Antibody \/ Netrin 4 is a anti-NTN4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NTN4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNTN4 antibody detects Netrin-4, a secreted extracellular matrix protein that guides axonal growth, angiogenesis, and tissue morphogenesis. The UniProt recommended name is Netrin-4 (NTN4). This laminin-related guidance molecule plays a dual role in promoting or inhibiting cell migration and adhesion depending on receptor context and tissue environment.\u003cbr\u003e\u003cbr\u003eFunctionally, NTN4 antibody identifies a 628-amino-acid protein secreted into the extracellular space, where it binds to receptors such as UNC5B, DCC, and integrins. NTN4 regulates neuronal guidance during development by controlling axonal attraction and repulsion. Beyond the nervous system, NTN4 influences angiogenesis by modulating endothelial cell proliferation and migration, acting as both a pro- and anti-angiogenic factor in different contexts. NTN4 also contributes to basement membrane organization and vascular permeability regulation.\u003cbr\u003e\u003cbr\u003eThe NTN4 gene is located on chromosome 12q22 and is expressed in brain, lung, kidney, and vascular tissues. Expression is upregulated during embryonic development, tissue regeneration, and pathological angiogenesis. NTN4 is also found in the extracellular matrix of epithelial and endothelial cells, where it interacts with laminins and collagen IV to maintain structural stability.\u003cbr\u003e\u003cbr\u003ePathologically, altered NTN4 expression is associated with cancer, diabetic retinopathy, and neurodevelopmental disorders. Overexpression can promote tumor growth and angiogenesis, while loss of function leads to impaired vessel formation and axonal misguidance. NTN4 acts as a context-dependent regulator of cellular adhesion, guiding both neural and vascular remodeling. Research using NTN4 antibody supports studies in extracellular matrix biology, neurodevelopment, and angiogenesis.\u003cbr\u003e\u003cbr\u003eNTN4 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect secreted matrix proteins. \u003cbr\u003e\u003cbr\u003eStructurally, Netrin-4 consists of a laminin N-terminal domain (LN), three laminin-type epidermal growth factor-like repeats (LE), and a C-terminal NTR domain that mediates receptor interactions. This modular organization enables NTN4 to function as both a structural matrix component and a signaling ligand. This antibody facilitates research into NTN4's roles in neural patterning, angiogenesis, and tumor microenvironment regulation.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NTN4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NTN4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NTN4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NTN4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NTN4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047321100653,"sku":"FY13278","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_487b1047-0350-4f32-a1a9-c9e56be9a53d.jpg?v=1782237082","url":"https:\/\/www.ebiohippo.com\/products\/ntn4-antibody-netrin-4-bha17136180","provider":"BioHippo","version":"1.0","type":"link"}