{"product_id":"nucleic-acid-removal-kit-bht16300172","title":"Nucleic Acid Removal Kit","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eProtein Purification\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eNucleic acids interfere with many steps of protein work, including cell lysis, chromatographic purification, protein quantification, activity assays and crystallization, especially when the protein of interest binds DNA or RNA. The Nucleic Acid Removal Kit removes nucleic acids from a protein solution or cell lysate to below 250 pg\/ml.\u003c\/p\u003e\n\u003cp\u003eNAR Reagent 1 and Reagent 2 are ionic polymers that precipitate nucleic acids, which are then removed by centrifugation. The final solution contains 0.45 M NaCl, and the reagents are easily removed during subsequent protein purification.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003e22 ml + 22 ml (for 200 ml sample) – NAR911\u003c\/th\u003e\n\u003ctd\u003e22 ml NAR Reagent 1 and 22 ml NAR Reagent 2, enough to treat 200 ml of cell lysate or protein solution\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponents\u003c\/th\u003e\n\u003ctd\u003e22 ml NAR Reagent 1, 22 ml NAR Reagent 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCapacity\u003c\/th\u003e\n\u003ctd\u003e200 ml of cell lysate or protein solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eResidual nucleic acid\u003c\/th\u003e\n\u003ctd\u003eBelow 250 pg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eFinal salt\u003c\/th\u003e\n\u003ctd\u003e0.45 M NaCl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eNAR911\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped cold (not frozen).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol Outline\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003eKeep all solutions at 0–4°C. Mix one volume of NAR Reagent 1 with 9 volumes of cell suspension (then lyse) or protein solution (then incubate on ice for 10 min).\u003c\/li\u003e\n\u003cli\u003eRemove insoluble material or precipitate by centrifugation at 6000 x g for 20 min at 4°C.\u003c\/li\u003e\n\u003cli\u003eAdd one volume of NAR Reagent 2, incubate on ice for 5 min and remove the precipitate by centrifugation at 6000 x g for 20 min at 4°C.\u003c\/li\u003e\n\u003cli\u003eContinue with protein purification; for ion exchange chromatography, dialyze against a low-salt buffer first.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe ProFoldin protocol “Nucleic Acid Removal” is linked under Documents.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"22 ml + 22 ml (for 200 ml sample)","offer_id":67828319486317,"sku":"NAR911","price":195.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/web.gif?v=1793129658","url":"https:\/\/www.ebiohippo.com\/products\/nucleic-acid-removal-kit-bht16300172","provider":"BioHippo","version":"1.0","type":"link"}