{"product_id":"nudt18-antibody-nudix-hydrolase-18-bha17135376","title":"NUDT18 Antibody \/ Nudix hydrolase 18","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNUDT18 Antibody \/ Nudix hydrolase 18 is a anti-NUDT18 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NUDT18\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNUDT18 antibody recognizes Nudix hydrolase 18, an enzyme that belongs to the Nudix hydrolase superfamily responsible for sanitizing the nucleotide pool by hydrolyzing potentially mutagenic or damaged nucleoside triphosphates. These hydrolases protect cells from incorporating oxidized nucleotides such as 8-oxo-dGTP into DNA, which could otherwise lead to mutations and genomic instability. NUDT18 specifically acts on ADP-ribose derivatives and oxidized nucleotide triphosphates, maintaining genomic integrity under conditions of oxidative stress. The NUDT18 antibody is widely used in studies of DNA repair, oxidative stress response, and metabolic regulation of nucleotide turnover.\u003cbr\u003e\u003cbr\u003eNUDT18 is encoded by the NUDT18 gene located on human chromosome 10q24.1. The protein contains the characteristic Nudix box motif (GX5EXEEAXE), which coordinates divalent metal ions and mediates catalysis. NUDT18 hydrolyzes substrates such as ADP-ribose and 8-oxo-dGDP, generating corresponding monophosphates and thereby removing potentially harmful oxidized nucleotide intermediates. It is localized in both cytoplasmic and mitochondrial compartments, indicating a role in protecting nucleic acid integrity in multiple cellular environments.\u003cbr\u003e\u003cbr\u003eThe NUDT18 antibody is typically used to detect a band near 35 kilodaltons on western blot, corresponding to the predicted molecular weight of the mature enzyme. Immunofluorescence analysis shows cytosolic and perinuclear localization, often associated with mitochondria-rich regions. NUDT18 expression is induced by oxidative agents and during cellular stress, suggesting a compensatory mechanism to prevent mutagenesis. Studies have shown that loss or suppression of NUDT18 increases susceptibility to oxidative DNA damage and enhances mutation frequency, linking it to cancer prevention and mitochondrial homeostasis.\u003cbr\u003e\u003cbr\u003eBeyond its antioxidant role, NUDT18 contributes to the regulation of ADP-ribose metabolism, a process associated with signaling pathways involving poly(ADP-ribose) polymerases (PARPs) and sirtuins. By degrading ADP-ribose derivatives, NUDT18 helps reset post-translational modification cycles that regulate DNA repair and apoptosis. Dysregulation of this enzyme has been implicated in metabolic stress responses, neurodegenerative diseases, and tumor biology.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NUDT18 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NUDT18 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NUDT18 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NUDT18 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NUDT18+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289413997,"sku":"FY12473","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_af59460f-80c9-4ea9-bf4f-be4140af8041.jpg?v=1782237013","url":"https:\/\/www.ebiohippo.com\/products\/nudt18-antibody-nudix-hydrolase-18-bha17135376","provider":"BioHippo","version":"1.0","type":"link"}