{"product_id":"nudt7-antibody-peroxisomal-coenzyme-a-diphosphatase-nudt7-bha17136126","title":"NUDT7 Antibody \/ Peroxisomal coenzyme A diphosphatase NUDT7","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNUDT7 Antibody \/ Peroxisomal coenzyme A diphosphatase NUDT7 is a anti-NUDT7 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Golgi, Nucleus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NUDT7\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNUDT7 antibody detects Peroxisomal coenzyme A diphosphatase NUDT7, an enzyme that regulates coenzyme A (CoA) and acyl-CoA metabolism within peroxisomes. The UniProt recommended name is Peroxisomal coenzyme A diphosphatase NUDT7 (NUDT7). This enzyme belongs to the Nudix hydrolase family, which hydrolyzes nucleoside diphosphates linked to various moieties, maintaining metabolic balance in oxidative organelles.\u003cbr\u003e\u003cbr\u003eFunctionally, NUDT7 antibody identifies a 282-amino-acid peroxisomal enzyme that hydrolyzes CoA, dephospho-CoA, and acyl-CoA derivatives into 3?,5?-ADP and the corresponding phosphopantetheine moiety. This activity helps regulate peroxisomal CoA levels, preventing CoA accumulation and maintaining optimal substrate availability for beta-oxidation and lipid metabolism. By modulating CoA turnover, NUDT7 indirectly influences energy metabolism, fatty acid oxidation, and reactive oxygen species detoxification.\u003cbr\u003e\u003cbr\u003eThe NUDT7 gene is located on chromosome 16p13.3 and is primarily expressed in liver and kidney, with additional expression in heart and skeletal muscle. Its activity is tightly regulated by nutritional status and metabolic signals such as fasting, reflecting its integration within energy homeostasis pathways.\u003cbr\u003e\u003cbr\u003ePathologically, altered NUDT7 activity can disrupt lipid metabolism and contribute to metabolic disorders. Reduced enzyme activity impairs peroxisomal CoA recycling, potentially leading to accumulation of acyl-CoA intermediates and oxidative stress. Overexpression has been linked to adaptive metabolic responses during fasting or high-fat diet conditions. Research using NUDT7 antibody supports studies in lipid metabolism, peroxisomal function, and metabolic regulation.\u003cbr\u003e\u003cbr\u003eNUDT7 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect peroxisomal enzymes. \u003cbr\u003e\u003cbr\u003eStructurally, Peroxisomal coenzyme A diphosphatase NUDT7 contains a conserved Nudix motif (GX5EX7REUXEEXGU) essential for divalent cation-dependent hydrolysis. The enzyme forms homodimers within peroxisomes, providing catalytic efficiency and substrate specificity. This antibody enables the study of NUDT7's role in peroxisomal metabolic control and its contribution to energy regulation in mammalian cells.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NUDT7 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NUDT7 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NUDT7 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NUDT7 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NUDT7+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047320641901,"sku":"FY13224","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ad0ef783-ddbb-4781-a8c2-4263fe92817e.jpg?v=1782237097","url":"https:\/\/www.ebiohippo.com\/products\/nudt7-antibody-peroxisomal-coenzyme-a-diphosphatase-nudt7-bha17136126","provider":"BioHippo","version":"1.0","type":"link"}