{"product_id":"nudt9-antibody-adp-ribose-pyrophosphatase-bha17135813","title":"NUDT9 Antibody \/ ADP-ribose pyrophosphatase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNUDT9 Antibody \/ ADP-ribose pyrophosphatase is a anti-NUDT9 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (Mitochondria).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NUDT9\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNUDT9 antibody detects ADP-ribose pyrophosphatase, a member of the nudix hydrolase family that hydrolyzes ADP-ribose into AMP and ribose-5-phosphate, maintaining intracellular nucleotide balance. Encoded by the NUDT9 gene on chromosome 4q22.1, this enzyme regulates ADP-ribose metabolism and contributes to NAD+ turnover, calcium signaling, and oxidative stress responses. NUDT9 ensures proper degradation of ADP-ribose derived from poly(ADP-ribose) polymerase (PARP) activity following DNA damage.\u003cbr\u003e\u003cbr\u003eStructurally, NUDT9 is a 350-amino-acid mitochondrial enzyme of approximately 40 kilodaltons containing a conserved Nudix box motif (GX5EXEEAXEE) that coordinates magnesium ions for catalytic hydrolysis of ADP-ribose. The enzyme localizes primarily to mitochondria and cytoplasm, where it prevents accumulation of free ADP-ribose that could perturb energy metabolism and calcium homeostasis. Its enzymatic activity helps recycle components of NAD+ metabolism and mitigate oxidative stress.\u003cbr\u003e\u003cbr\u003eThe NUDT9 antibody is widely used in biochemistry, metabolism, and mitochondrial biology research to study nucleotide turnover, ADP-ribose signaling, and cellular energy regulation. Western blot analysis detects a 40 kilodalton band corresponding to NUDT9, while immunofluorescence reveals mitochondrial and cytosolic localization. This antibody supports studies exploring enzymatic regulation of NAD+ pathways and mitochondrial nucleotide dynamics.\u003cbr\u003e\u003cbr\u003eFunctionally, NUDT9 plays a protective role against oxidative stress by regulating levels of ADP-ribose, a molecule that can activate TRPM2 channels and trigger calcium influx during stress responses. Dysregulation of NUDT9 expression affects calcium signaling, redox balance, and cell viability. Homologous enzymes and domains, such as those found in the TRPM2 channel, share structural similarity with NUDT9 and extend its functional relevance to ion channel regulation. The NUDT9 antibody provides a sensitive reagent for investigating nucleotide metabolism, mitochondrial stress adaptation, and PARP-related signaling.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NUDT9 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NUDT9 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NUDT9 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NUDT9 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NUDT9+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047307141485,"sku":"FY12911","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_40ce8a58-0a31-4430-8734-1161784fc493.jpg?v=1782237073","url":"https:\/\/www.ebiohippo.com\/products\/nudt9-antibody-adp-ribose-pyrophosphatase-bha17135813","provider":"BioHippo","version":"1.0","type":"link"}