{"product_id":"nup50-antibody-nucleoporin-50-bha17135339","title":"NUP50 Antibody \/ Nucleoporin 50","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNUP50 Antibody \/ Nucleoporin 50 is a anti-NUP50 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NUP50\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe NUP50 antibody targets Nuclear pore complex protein Nup50, a nuclear transport factor encoded by the NUP50 gene. Nuclear pore complex protein Nup50 is a dynamic component of the nuclear pore complex (NPC), where it functions in importin-alpha recycling and nuclear import termination. By interacting with importin-beta, RanGTP, and the nucleoporin Nup153, Nup50 promotes the release of import cargo into the nucleoplasm. The NUP50 antibody allows detailed examination of nucleocytoplasmic trafficking, NPC composition, and nuclear transport regulation.\u003cbr\u003e\u003cbr\u003eNuclear pore complex protein Nup50 belongs to the FG (phenylalanine-glycine) repeat�containing family of nucleoporins that mediate selective permeability within the nuclear pore. It localizes to the nuclear basket region of the NPC, where it acts as a cofactor in cargo disassembly and receptor recycling. The NUP50 antibody supports visualization of its distribution along the inner nuclear envelope and nucleoplasmic face, highlighting its mobility during interphase and mitosis. Its high turnover reflects an adaptable role in transport regulation and chromatin organization.\u003cbr\u003e\u003cbr\u003eBeyond its role in nuclear transport, Nuclear pore complex protein Nup50 has been implicated in gene regulation. It interacts with chromatin and transcription factors, suggesting a link between nuclear import and transcriptional control. The NUP50 antibody facilitates studies of this regulatory function, allowing researchers to explore how Nup50 coordinates transport with nuclear gene expression. Nup50 depletion alters transcriptional activity and nucleoplasmic organization, leading to cell-cycle arrest and reduced proliferation.\u003cbr\u003e\u003cbr\u003eDefects in NUP50 expression have been associated with neurodevelopmental and neuromuscular disorders. Mutations or reduced expression may impair nuclear transport of essential regulatory proteins. The NUP50 antibody supports investigations into these pathologies, enabling assessment of expression and localization in neuronal and muscle tissues. It also serves as a useful marker for nuclear pore integrity in aging and neurodegenerative models.\u003cbr\u003e\u003cbr\u003eThe NUP50 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, showing characteristic nuclear-rim and nucleoplasmic staining.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NUP50 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NUP50 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NUP50 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NUP50 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NUP50+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047288529261,"sku":"FY12436","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_cc29f33b-0568-4089-a53a-438ea1d1846f.jpg?v=1782237003","url":"https:\/\/www.ebiohippo.com\/products\/nup50-antibody-nucleoporin-50-bha17135339","provider":"BioHippo","version":"1.0","type":"link"}