{"product_id":"nup62-antibody-nucleoporin-62-bha17135468","title":"NUP62 Antibody \/ Nucleoporin 62","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNUP62 Antibody \/ Nucleoporin 62 is a anti-NUP62 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB), FACS (Intracellular) with listed reactivity in Human, Mouse. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NUP62\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, IF, IHC, ICC, WB, FACS (Intracellular)\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNUP62 antibody detects Nucleoporin 62, a central channel component of the nuclear pore complex that mediates selective nucleocytoplasmic transport of macromolecules. NUP62 forms part of the symmetrical ring structure and contributes to the permeability barrier that controls RNA export and protein import. The NUP62 antibody is used in molecular biology, nuclear transport, and cell cycle research to investigate nuclear envelope dynamics and transport regulation.\u003cbr\u003e\u003cbr\u003eNUP62 is encoded by the NUP62 gene located on human chromosome 19q13.32. The protein is approximately 520 amino acids long and contains multiple phenylalanine-glycine repeat motifs that create the selective barrier of the pore. NUP62 interacts with importins, exportins, and Ran GTPase to regulate directionality of cargo transport across the nuclear envelope.\u003cbr\u003e\u003cbr\u003eThe NUP62 antibody detects a 62 kilodalton band on western blot and shows punctate nuclear envelope staining by immunofluorescence. NUP62 forms a subcomplex with NUP54 and NUP58 to create the central transport channel, undergoing dynamic conformational changes during translocation events. This structure selectively allows passage of karyopherin-bound cargo while excluding non-specific diffusion.\u003cbr\u003e\u003cbr\u003eBeyond transport, NUP62 participates in mitosis, where portions of the nuclear pore disassemble to allow spindle formation and chromosome segregation. Mutations or mislocalization of NUP62 disrupt nuclear-cytoplasmic communication, leading to developmental defects and neurodegeneration. Autoantibodies against NUP62 have been identified in certain autoimmune diseases, reflecting its immunogenicity when nuclear integrity is compromised.\u003cbr\u003e\u003cbr\u003eThrough its integral role in nuclear transport and cell cycle progression, NUP62 maintains genome organization and communication between nuclear and cytoplasmic compartments.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NUP62 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NUP62 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NUP62 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NUP62 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NUP62+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047293084013,"sku":"FY12565","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_b6853903-e6eb-4b01-b75e-5214b3a25d30.jpg?v=1782237020","url":"https:\/\/www.ebiohippo.com\/products\/nup62-antibody-nucleoporin-62-bha17135468","provider":"BioHippo","version":"1.0","type":"link"}