{"product_id":"nup98-antibody-nucleoporin-98-bha17135761","title":"NUP98 Antibody \/ Nucleoporin 98","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNUP98 Antibody \/ Nucleoporin 98 is a anti-NUP98 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Monkey, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NUP98\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNUP98 antibody detects Nuclear pore complex protein Nup98, a critical nucleoporin involved in nucleocytoplasmic transport, gene regulation, and mitotic progression. Encoded by the NUP98 gene on chromosome 11p15.4, this large modular protein forms part of the nuclear pore complex (NPC), which mediates bidirectional transport of macromolecules between the nucleus and cytoplasm. Nup98 functions not only as a structural component of the NPC but also as a dynamic regulator of gene expression and chromatin organization.\u003cbr\u003e\u003cbr\u003eNup98 contains FG (phenylalanine-glycine)-repeat domains that form flexible meshwork structures facilitating selective nucleocytoplasmic transport. The C-terminal region anchors to the NPC scaffold via interactions with other nucleoporins such as Nup96 and Nup88. Beyond its classical transport role, Nup98 shuttles between the nuclear envelope and nucleoplasm, where it binds transcriptional regulators, influencing developmental and immune response genes. It also participates in RNA export, mRNA surveillance, and mitotic spindle assembly.\u003cbr\u003e\u003cbr\u003eThe NUP98 antibody is widely used in cell biology, oncology, and molecular genetics research to study nuclear transport, chromatin organization, and cell cycle regulation. Western blot analysis identifies a 98 kilodalton band corresponding to Nup98, while immunofluorescence shows punctate staining at the nuclear envelope and within nucleoplasmic foci. This antibody supports studies examining NPC function, transcriptional regulation, and nuclear architecture integrity.\u003cbr\u003e\u003cbr\u003eTranslocations involving NUP98 are recurrent in acute myeloid leukemia (AML) and myelodysplastic syndromes (MDS), producing oncogenic Nup98 fusion proteins such as NUP98-HOXA9 and NUP98-NSD1. These fusions retain the FG-repeat domain of Nup98 but acquire transcriptional activation capabilities from their fusion partners, leading to deregulated gene expression and leukemogenesis. The NUP98 antibody provides a reliable tool for studying such fusion proteins and their oncogenic mechanisms.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NUP98 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NUP98 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NUP98 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NUP98 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NUP98+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047304388973,"sku":"FY12859","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_951ec21b-fdf1-4743-bf61-3712b64b9b38.jpg?v=1782237048","url":"https:\/\/www.ebiohippo.com\/products\/nup98-antibody-nucleoporin-98-bha17135761","provider":"BioHippo","version":"1.0","type":"link"}