{"product_id":"nupr1-antibody-nuclear-protein-1-bha17135790","title":"NUPR1 Antibody \/ Nuclear protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNUPR1 Antibody \/ Nuclear protein 1 is a anti-NUPR1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NUPR1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNUPR1 antibody detects Nuclear protein 1, a small chromatin-associated transcriptional regulator induced by cellular stress. Encoded by the NUPR1 gene on chromosome 16p11.2, this intrinsically disordered nuclear protein is activated in response to stress stimuli such as oxidative damage, hypoxia, and nutrient deprivation. NUPR1 modulates gene expression related to cell cycle control, apoptosis, and autophagy, playing a pivotal role in stress adaptation and tumor progression.\u003cbr\u003e\u003cbr\u003eStructurally, NUPR1 is a 100-amino-acid protein of approximately 9 kilodaltons lacking defined secondary structure, which enables flexible interactions with DNA and chromatin-modifying enzymes. It acts as a transcriptional cofactor rather than a classical DNA-binding protein, partnering with p300, SMADs, and histone modifiers to influence transcriptional responses. NUPR1 localizes predominantly to the nucleus but can shuttle between compartments depending on stress conditions and post-translational modifications.\u003cbr\u003e\u003cbr\u003eThe NUPR1 antibody is widely used in oncology, stress biology, and transcription research to study adaptive gene regulation and tumor cell survival. Western blot analysis detects a 9 kilodalton band corresponding to NUPR1, while immunofluorescence demonstrates strong nuclear localization that increases following stress induction. This antibody enables analysis of NUPR1 expression during oxidative and metabolic stress and its contribution to stress-responsive transcription networks.\u003cbr\u003e\u003cbr\u003eFunctionally, NUPR1 promotes survival under cytotoxic conditions by regulating genes involved in autophagy and anti-apoptotic pathways. It acts downstream of ATF4 and CHOP in the endoplasmic reticulum stress response and influences cell fate decisions by modulating p53-dependent transcription. In cancer, overexpression of NUPR1 supports tumor growth, resistance to chemotherapy, and epithelial-mesenchymal transition (EMT). Conversely, NUPR1 depletion sensitizes tumor cells to oxidative damage and metabolic inhibitors. The NUPR1 antibody provides a key research tool for exploring stress signaling, transcriptional plasticity, and tumor adaptability.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NUPR1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NUPR1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NUPR1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NUPR1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NUPR1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047304946029,"sku":"FY12888","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_942e533b-03df-4af7-9a06-db6c26547ed1.jpg?v=1782237061","url":"https:\/\/www.ebiohippo.com\/products\/nupr1-antibody-nuclear-protein-1-bha17135790","provider":"BioHippo","version":"1.0","type":"link"}