{"product_id":"nusap1-antibody-nucleolar-and-spindle-associated-protein-1-bha17135975","title":"NUSAP1 Antibody \/ Nucleolar and spindle-associated protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eNUSAP1 Antibody \/ Nucleolar and spindle-associated protein 1 is a anti-NUSAP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS) with listed reactivity in Human. Reported localization: Nucleus, Nucleolus.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e NUSAP1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eNUSAP1 antibody detects Nucleolar and spindle-associated protein 1, a microtubule-binding protein essential for spindle formation and chromosome segregation during mitosis. The UniProt recommended name is Nucleolar and spindle-associated protein 1 (NUSAP1). This mitotic regulator links chromatin to microtubules, ensuring proper spindle assembly and stabilization required for accurate chromosome alignment and segregation.\u003cbr\u003e\u003cbr\u003eFunctionally, NUSAP1 antibody identifies a 495-amino-acid nuclear protein that localizes to the mitotic spindle and spindle poles. NUSAP1 binds microtubules through its C-terminal domain and bundles them into stable arrays, promoting bipolar spindle formation. It also interacts with chromatin components, including DNA and nucleolar proteins, to coordinate chromosome condensation and segregation during metaphase and anaphase.\u003cbr\u003e\u003cbr\u003eThe NUSAP1 gene, located on chromosome 15q15.1, encodes a cell cycle-regulated protein that peaks in expression during G2\/M phase and is degraded after mitosis. NUSAP1 is phosphorylated by CDK1, controlling its localization and activity. It functions in concert with kinesin and dynein motors, contributing to spindle microtubule dynamics and mitotic checkpoint signaling.\u003cbr\u003e\u003cbr\u003ePathologically, elevated NUSAP1 expression is a hallmark of proliferating cells and various cancers, including breast, prostate, and lung carcinoma. Its overexpression correlates with increased tumor aggressiveness and poor prognosis, reflecting enhanced proliferative capacity. Conversely, NUSAP1 depletion leads to spindle defects, mitotic arrest, and genomic instability. Research with NUSAP1 antibody provides insight into cell division mechanisms and cancer cell cycle regulation.\u003cbr\u003e\u003cbr\u003eNUSAP1 antibody is suitable for use in western blotting, immunofluorescence, and immunohistochemistry to detect mitotic spindles and cell cycle progression. \u003cbr\u003e\u003cbr\u003eStructurally, NUSAP1 contains a coiled-coil domain, a microtubule-binding motif, and a DNA-binding region, which together mediate its dual role in chromatin attachment and microtubule bundling. This antibody supports exploration of NUSAP1�s role as a spindle scaffold that integrates chromosomal and cytoskeletal control during mitosis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=NUSAP1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=NUSAP1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=NUSAP1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/NUSAP1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=NUSAP1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047311696237,"sku":"FY13073","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ddaa5db0-d23f-48d1-879d-41d9d5751489.jpg?v=1772019431","url":"https:\/\/www.ebiohippo.com\/products\/nusap1-antibody-nucleolar-and-spindle-associated-protein-1-bha17135975","provider":"BioHippo","version":"1.0","type":"link"}