{"product_id":"oas2-antibody-2-5-oligoadenylate-synthetase-2-bha17136291","title":"Oas2 Antibody \/ 2-5-Oligoadenylate synthetase 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eOas2 Antibody \/ 2-5-Oligoadenylate synthetase 2 is a anti-OAS2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e OAS2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eOAS2 antibody detects 2'-5'-oligoadenylate synthetase 2, a double-stranded RNA-activated enzyme encoded by the OAS2 gene located on chromosome 12q24.13. OAS2 plays a central role in the innate immune response to viral infection by synthesizing 2'-5'-linked oligoadenylates (2-5A) upon activation by viral double-stranded RNA. These 2-5A molecules subsequently activate RNase L, leading to degradation of viral and cellular RNA and inhibition of viral replication. OAS2 is expressed in most tissues but is strongly upregulated in response to interferon signaling, particularly in immune cells such as macrophages, dendritic cells, and lymphocytes.\u003cbr\u003e\u003cbr\u003eStructurally, OAS2 is a 113 kDa protein containing two functional OAS catalytic domains, allowing higher polymerization activity than other family members such as OAS1. It belongs to the 2'-5'-oligoadenylate synthetase family of nucleotidyltransferases, characterized by a conserved polymerase fold and dsRNA-binding motifs. OAS2 localizes to the cytoplasm, often associating with ribosomes and endoplasmic reticulum membranes where viral replication typically occurs.\u003cbr\u003e\u003cbr\u003eFunctionally, OAS2 acts as a critical antiviral effector enzyme in interferon-stimulated pathways. Upon recognition of viral RNA, OAS2 catalyzes ATP polymerization into 2-5A molecules that bind and activate latent RNase L. This leads to RNA cleavage, apoptosis of infected cells, and amplification of antiviral signaling. OAS2 also interacts with pattern recognition receptors such as MDA5 and RIG-I, coordinating interferon response cascades. In addition to antiviral defense, OAS2 influences immune regulation, apoptosis, and inflammatory cytokine expression.\u003cbr\u003e\u003cbr\u003eOAS2 expression is strongly induced by type I and type III interferons. Genetic variants in OAS2 have been associated with altered susceptibility to viral infections including SARS-CoV-2, hepatitis C, and West Nile virus. Dysregulation of OAS2 can contribute to autoimmune disorders through excessive RNA degradation. Pathway associations include interferon signaling, RNA decay, and innate immune activation. During development, OAS2 expression in immune progenitors supports early antiviral competence.\u003cbr\u003e\u003cbr\u003eThe OAS2 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=OAS2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=OAS2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=OAS2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/OAS2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=OAS2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047324279149,"sku":"FY13390","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6caeac58-51e7-4ca9-ae89-464b200f3f1f.jpg?v=1782237110","url":"https:\/\/www.ebiohippo.com\/products\/oas2-antibody-2-5-oligoadenylate-synthetase-2-bha17136291","provider":"BioHippo","version":"1.0","type":"link"}