{"product_id":"odf1-antibody-outer-dense-fiber-protein-1-bha17135217","title":"ODF1 Antibody \/ Outer dense fiber protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eODF1 Antibody \/ Outer dense fiber protein 1 is a anti-ODF1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ODF1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eODF1 antibody detects Outer dense fiber protein 1, encoded by the ODF1 gene on chromosome 8q22.2. ODF1 antibody is widely applied in reproductive biology, spermatogenesis, and fertility research. ODF1 is a structural protein of the sperm tail, specifically localized to the outer dense fibers that run alongside the axoneme in the principal piece of the flagellum. These fibers provide mechanical strength, protect the axoneme from shear stress, and contribute to the elastic recoil properties of sperm movement.\u003cbr\u003e\u003cbr\u003eStructurally, ODF1 is a ~27 kDa coiled-coil protein with leucine zipper motifs that promote oligomerization. It is one of the earliest proteins incorporated into outer dense fibers during spermatid differentiation. ODF1 interacts with other outer dense fiber proteins such as ODF2 and with axonemal microtubules, forming the cytoskeletal backbone of the sperm tail.\u003cbr\u003e\u003cbr\u003eFunctionally, ODF1 contributes to sperm motility by reinforcing the axoneme and supporting energy transmission along the flagellum. Loss of ODF1 leads to structural abnormalities in the sperm tail and impaired motility, resulting in infertility. Researchers use ODF1 antibody to study spermatogenesis, sperm structure, and male fertility.\u003cbr\u003e\u003cbr\u003eClinically, ODF1 mutations or dysregulation have been associated with asthenozoospermia and male infertility. Studies in mice show that ODF1 knockout results in male sterility due to defective sperm tails. Because sperm tail defects are a common cause of infertility, ODF1 is a biomarker of reproductive health. \u003cbr\u003e\u003cbr\u003eExperimentally, ODF1 antibody is used in western blotting to detect the ~27 kDa protein, in immunofluorescence to visualize sperm tail localization, and in immunohistochemistry of testicular sections. Co-immunoprecipitation with ODF1 antibody reveals interactions with cytoskeletal partners critical for flagellar integrity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ODF1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ODF1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ODF1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ODF1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ODF1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047284564333,"sku":"FY12314","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_20a2645d-3461-4aa8-af7d-9cec3e1b26d9.jpg?v=1782236988","url":"https:\/\/www.ebiohippo.com\/products\/odf1-antibody-outer-dense-fiber-protein-1-bha17135217","provider":"BioHippo","version":"1.0","type":"link"}