{"product_id":"ogfod3-antibody-2-oxoglutarate-and-iron-dependent-oxygenase-domain-containing-protein-3-bha17135612","title":"OGFOD3 Antibody \/ 2-Oxoglutarate and iron-dependent oxygenase domain-containing protein 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eOGFOD3 Antibody \/ 2-Oxoglutarate and iron-dependent oxygenase domain-containing protein 3 is a anti-OGFOD3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e OGFOD3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eOGFOD3 antibody detects 2-oxoglutarate and iron-dependent oxygenase domain-containing protein 3, a member of the 2OG\/Fe(II)-dependent dioxygenase superfamily involved in protein hydroxylation and cellular stress regulation. Encoded by the OGFOD3 gene on chromosome 1p22.2, this small nuclear protein contains a conserved dioxygenase domain that utilizes 2-oxoglutarate and Fe2+ as cofactors for oxidative catalysis. Although its exact substrates remain under investigation, OGFOD3 is thought to participate in hydroxylation of ribosomal and transcriptional proteins, influencing translation fidelity and stress responses. This family of enzymes links oxygen and nutrient availability to cellular metabolism and gene expression through oxygen-dependent modification reactions.\u003cbr\u003e\u003cbr\u003eOGFOD3 is expressed in most tissues, with notable enrichment in the brain, testis, and liver. Localization studies show nuclear distribution with occasional cytoplasmic presence, suggesting roles in both nuclear signaling and translational control. Comparative genomics indicates that OGFOD3 shares structural similarity with OGFOD1, an enzyme known to hydroxylate ribosomal protein RPS23, implying potential overlap in function. The OGFOD3 antibody is used to investigate dioxygenase-mediated post-translational modifications and the cellular response to oxidative or metabolic stress.\u003cbr\u003e\u003cbr\u003eAlthough less characterized than its paralogs, OGFOD3 has been linked to cellular stress pathways and protein biosynthesis. Studies show that depletion of OGFOD3 alters ribosomal activity and activates integrated stress response signaling. In cancer research, OGFOD3 expression is dysregulated in several tumor types, including lung and colon carcinomas, where it may influence adaptation to hypoxia or nutrient deprivation. The OGFOD3 antibody allows researchers to detect expression changes under stress conditions and to localize the protein within cells using immunocytochemical or immunofluorescent assays. Western blot analysis identifies a 38 kilodalton band corresponding to the full-length protein.\u003cbr\u003e\u003cbr\u003eIn the context of oxygen sensing and translational control, OGFOD3 may coordinate with other 2OG oxygenases to maintain homeostasis during metabolic shifts. It potentially affects protein synthesis by modifying translation factors or ribosomal subunits, modulating global translational output in response to stress.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=OGFOD3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=OGFOD3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=OGFOD3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/OGFOD3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=OGFOD3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047297212781,"sku":"FY12709","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6e914789-a38c-4d69-aed5-b011d585aa9c.jpg?v=1782237036","url":"https:\/\/www.ebiohippo.com\/products\/ogfod3-antibody-2-oxoglutarate-and-iron-dependent-oxygenase-domain-containing-protein-3-bha17135612","provider":"BioHippo","version":"1.0","type":"link"}