{"product_id":"one-step-probe-rt-pcr-mix-bht15200046","title":"One Step Probe RT-PCR Mix","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eOne-Step RT-PCR Mixes\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis product is a special reagent for Real Time One Step RT-PCR using the probe method. Using this product for Real Time RT-PCR reaction can continuously perform reverse transcription and PCR amplification in the same reaction tube. It is simple to operate and can effectively prevent contamination. Since this reaction system can monitor the amplified products in real time, the detection sensitivity is greatly improved, and the electrophoresis step after PCR reaction is omitted, which is very suitable for the detection of RNA viruses. This product uses high-efficiency reverse transcriptase and high-specificity hot-start Taq DNA polymerase to perform stable and efficient Real Time One Step RT-PCR reactions. For the fluorescent quantitative PCR instrument that uses ROX as the calibration dye, this product is equipped with a separate ROX dye to correct the fluorescent signal error generated between the wells of the quantitative PCR instrument.\u003c\/p\u003e\n\u003cp\u003eTemplate RNA input is 1 pg–1 µg per reaction, reaction volumes of 20 µL or 50 µL are recommended and amplified products are 80–250 bp. The program is reverse transcription at 50°C for 15 min, pre-denaturation at 95°C for 2 min, then 35–40 cycles of 95°C for 10 sec and 60–65°C for 30 sec.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003e2× one-step RT-PCR mix with separate RT-PCR Enzyme Mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection chemistry\u003c\/th\u003e\n\u003ctd\u003eProbe method\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReverse transcriptase\u003c\/th\u003e\n\u003ctd\u003eHigh-efficiency third-generation reverse transcriptase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePolymerase\u003c\/th\u003e\n\u003ctd\u003eHigh-specificity hot-start Taq DNA polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eROX\u003c\/th\u003e\n\u003ctd\u003eSeparate 50× ROX Dye supplied\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProbe and primers\u003c\/th\u003e\n\u003ctd\u003eNot supplied; labelled probe and primers added by the user\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTemplate input\u003c\/th\u003e\n\u003ctd\u003eRNA 1 pg–1 µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReaction volume\u003c\/th\u003e\n\u003ctd\u003e20 µL or 50 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAmplified product size\u003c\/th\u003e\n\u003ctd\u003e80–250 bp\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e20 µL × 100 rxn\u003c\/th\u003e\n\u003cth\u003e20 µL × 500 rxn\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2×One Step RT-PCR Mix (Probe)\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003ctd\u003e1.25 mL × 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRT-PCR Enzyme Mix\u003c\/td\u003e\n\u003ctd\u003e150 µL\u003c\/td\u003e\n\u003ctd\u003e750 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e50×ROX Dye\u003c\/td\u003e\n\u003ctd\u003e250 µL\u003c\/td\u003e\n\u003ctd\u003e1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-free ddH2O\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003ctd\u003e1.25 mL × 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eReal-time one-step RT-PCR using the probe method\u003c\/li\u003e\n\u003cli\u003eDetection of viral RNA in research samples\u003c\/li\u003e\n\u003cli\u003eDetection of trace RNA\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAdd RNA only: reverse transcription and PCR amplification run consecutively in the same PCR tube, keeping the operation simple.\u003c\/li\u003e\n\u003cli\u003eReal-time detection of amplified products greatly improves detection sensitivity and can effectively prevent contamination.\u003c\/li\u003e\n\u003cli\u003eThe preferred third-generation reverse transcriptase is used in conjunction with the hot-start Taq enzyme to obtain optimal amplification and effectively reduce non-specific amplification.\u003c\/li\u003e\n\u003cli\u003eThe electrophoresis step after the PCR reaction is omitted, which is very suitable for the detection of trace RNA.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eAll components are stored at -20°C with a shelf life of 2 years for both sizes. Avoid strong light.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eDo not vortex the reagents.\u003c\/li\u003e\n\u003cli\u003eAvoid repeated freeze-thaw cycles.\u003c\/li\u003e\n\u003cli\u003eAvoid strong light.\u003c\/li\u003e\n\u003cli\u003eUse filter tips and keep reagents on ice.\u003c\/li\u003e\n\u003cli\u003eMinimize contamination risk during setup.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEQ015\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200046\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"20 uL x 100 rxn","offer_id":53965512933741,"sku":"EQ015-100RXN","price":182.0,"currency_code":"USD","in_stock":true},{"title":"20 uL x 500 rxn","offer_id":53965516439917,"sku":"EQ015-500RXN","price":624.0,"currency_code":"USD","in_stock":true},{"title":"20 uL x 100 rxn x 10","offer_id":53965516472685,"sku":"EQ015-100RXNX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"20 uL x 500 rxn x 4","offer_id":53965516505453,"sku":"EQ015-500RXNX4","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EQ015-a-1.jpg?v=1789419324","url":"https:\/\/www.ebiohippo.com\/products\/one-step-probe-rt-pcr-mix-bht15200046","provider":"BioHippo","version":"1.0","type":"link"}