{"product_id":"onescript-hot-cdna-synthesis-kit-bht10900007","title":"OneScript® Hot cDNA Synthesis Kit","description":"\u003cp\u003e\u003cstrong\u003eOneScript\u003csup\u003e®\u003c\/sup\u003e Hot cDNA Synthesis Kit\u003c\/strong\u003e is the first kit in the world that allows for efficient and robust cDNA synthesis at 60ºC. All other cDNA synthesis kits on the market are optimized for the reaction to be performed at 42-55ºC, which is sufficient for most RNA template, but fails on more difficult templates. \u003cstrong\u003eabm \u003c\/strong\u003eis the only company in the world to have a reverse transcriptase engineered to offer superior cDNA synthesis performance with even the most challenging RNA samples due to its\u003cstrong\u003e incredible thermostability at 60-72ºC.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe enzyme is a mutational derivative of Moloney-Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) that efficiently reverse transcribes low-abundance or partially degraded RNA and exhibits enhanced resistance to common inhibitors such as reagents used during RNA extraction and contaminants from biological samples. It features genetic modifications that abolish RNase H activity to achieve thermal stability, allowing the RNA template to remain intact during first-strand synthesis for higher cDNA yield and fidelity. Its high processivity and sensitivity enable rapid synthesis of full-length cDNA fragments in a fraction of the time required by leading competitors. OneScript® Hot is formulated with \u003cstrong\u003eabm\u003c\/strong\u003e’s RNaseOFF Ribonuclease Inhibitor, which provides improved resistance to oxidation compared to traditional, oxidation-sensitive human RNase inhibitors. RNaseOFF remains stable even under very low concentrations of DTT (\u0026lt;1 mM), ensuring superior RNA protection and reliability in demanding experimental conditions.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Features:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eExtremely thermostable cDNA synthesis at 60-72ºC\u003c\/li\u003e\n\u003cli\u003eTranscribes degraded samples and resists inhibitors\/contaminants\u003c\/li\u003e\n\u003cli\u003eHigh cDNA yields from difficult or ultra-low RNA samples\u003c\/li\u003e\n\u003cli\u003eIncluded in \u003cstrong\u003eabm\u003c\/strong\u003e's \u003ca\u003ePCR Buffet Program\u003c\/a\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"margin: 30px 0;\"\u003e\u003c\/p\u003e\n\u003ctable class=\"bootstrap-table bootstrap-table-hover abm-service-table abm-perfect-table2\" style=\"margin-bottom: 20px;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003e\u003cstrong\u003eProduct Component\u003c\/strong\u003e\u003c\/th\u003e\n\u003cth\u003e\u003cstrong\u003eQuantity\u003c\/strong\u003e\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctfoot\u003e\n\u003ctr\u003e\n\u003ctd\u003eOneScript\u003csup\u003e®\u003c\/sup\u003e Hot Reverse Transcriptase\u003c\/td\u003e\n\u003ctd\u003e100 rxn (100 µl)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5X RT Buffer\u003c\/td\u003e\n\u003ctd\u003e400 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOligo(dT) (10 μM)\u003c\/td\u003e\n\u003ctd\u003e100 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRandom Primers (10 μM)\u003c\/td\u003e\n\u003ctd\u003e100 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003edNTP (10 mM)\u003c\/td\u003e\n\u003ctd\u003e100 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNuclease-Free H\u003csub\u003e2\u003c\/sub\u003eO\u003c\/td\u003e\n\u003ctd\u003e2 x 1.0 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tfoot\u003e\n\u003c\/table\u003e\n\u003ctable style=\"border-collapse: collapse; background-color: #f9eae4; border-radius: 10px; text-align: center; width: 100%;\" border=\"0\" cellpadding=\"10\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"vertical-align: middle;\"\u003e\n\u003ctd style=\"text-align: left; vertical-align: middle;\" width=\"15%\"\u003e\u003cimg src=\"\/assets\/images\/wysiwyg\/ISO-Logo-v1.png\" alt=\"ISO 13485:2016 MDSAP Certified\" width=\"85%\"\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 20px 20px 20px 0; text-align: left; vertical-align: middle;\"\u003e\n\u003cp\u003e\u003cstrong\u003eISO 13485:2016 MDSAP Certified\u003c\/strong\u003e\u003cbr\u003eOur PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ctable style=\"border-collapse: collapse; background-color: #f4f4f4; border-radius: 10px; text-align: center; width: 100%; margin-top: 20px;\" border=\"0\" cellpadding=\"10\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"vertical-align: middle;\"\u003e\n\u003ctd style=\"padding: 20px; text-align: center; vertical-align: middle;\"\u003e\n\u003cp\u003eThis product is equivalent to \u003cstrong\u003eRScript Hot CDNA Synthesis Kit (Cat. No. G594EU)\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"100 rxn","offer_id":53311769739629,"sku":"G594","price":207.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/g593-onescript-hot-reverse-transcriptase-product-photo_1.png?v=1778555685","url":"https:\/\/www.ebiohippo.com\/products\/onescript-hot-cdna-synthesis-kit-bht10900007","provider":"BioHippo","version":"1.0","type":"link"}