{"product_id":"opn3-antibody-opsin-3-bha17135951","title":"OPN3 Antibody \/ Opsin 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eOPN3 Antibody \/ Opsin 3 is a anti-OPN3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e OPN3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eOPN3 antibody detects Opsin-3, a light-sensitive G protein-coupled receptor (GPCR) that belongs to the opsin subfamily of photoreceptors. The UniProt recommended name is Opsin-3 (OPN3). This protein, also known as encephalopsin or panopsin, functions as a non-visual opsin that may mediate light-sensitive signaling in neural and peripheral tissues.\u003cbr\u003e\u003cbr\u003eFunctionally, OPN3 antibody identifies a 402-amino-acid seven-transmembrane GPCR that binds retinal chromophore and activates intracellular signaling pathways in response to light or metabolic cues. OPN3 couples to heterotrimeric G proteins, influencing cyclic nucleotide levels, calcium signaling, and other downstream responses. Its activity extends beyond traditional photoreception, integrating environmental light signals into physiological regulation of circadian rhythm, metabolism, and hormone secretion.\u003cbr\u003e\u003cbr\u003eThe OPN3 gene is located on chromosome 1q43 and encodes a protein expressed in brain, skin, testis, and adipose tissue. OPN3 is localized primarily in cell membranes and intracellular vesicles, suggesting context-dependent signaling functions. In the brain, it may modulate neuronal excitability and metabolic responses to light exposure. In skin, OPN3 contributes to phototransduction in melanocytes and keratinocytes, influencing pigmentation and UV response.\u003cbr\u003e\u003cbr\u003eEmerging evidence implicates OPN3 in non-visual light sensing that affects circadian entrainment, energy homeostasis, and mood regulation. It may act as a photoreceptor for near-infrared or blue light, mediating cellular adaptation to environmental illumination. In metabolic tissues, OPN3 modulates lipid metabolism and thermogenesis, linking light exposure to systemic energy balance. OPN3 has also been studied for its potential role in reproductive physiology and skin photobiology.\u003cbr\u003e\u003cbr\u003eOPN3 antibody is widely used in photoreceptor biology, neuroendocrinology, and GPCR signaling research. It is suitable for immunofluorescence, western blotting, and immunohistochemistry to detect OPN3 localization in neural, skin, and endocrine tissues. This antibody supports research into light-mediated signaling, opsin evolution, and photoregulated physiology. In biomedical studies, OPN3 serves as a molecular link between light sensing and metabolic control.\u003cbr\u003e\u003cbr\u003eStructurally, OPN3 exhibits the canonical GPCR topology with seven transmembrane helices, an extracellular retinal-binding lysine, and cytoplasmic domains that interact with G proteins.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=OPN3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=OPN3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=OPN3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/OPN3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=OPN3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047310418285,"sku":"FY13049","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_8e80e5d4-776f-4f12-99f5-4ef04fc658ec.jpg?v=1782237094","url":"https:\/\/www.ebiohippo.com\/products\/opn3-antibody-opsin-3-bha17135951","provider":"BioHippo","version":"1.0","type":"link"}