{"product_id":"opn5-antibody-opsin-5-neuropsin-bha17135655","title":"OPN5 Antibody \/ Opsin 5 \/ Neuropsin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eOPN5 Antibody \/ Opsin 5 \/ Neuropsin is a anti-OPN5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e OPN5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eOPN5 antibody detects Opsin-5 (also known as Neuropsin or G protein-coupled receptor 136), a light-sensitive G protein-coupled receptor involved in non-visual photoreception. Encoded by the OPN5 gene on chromosome 6p12.3, this protein belongs to the opsin family of photoreceptive GPCRs that use retinaldehyde chromophores to detect light. Opsin-5 absorbs near-ultraviolet light and activates intracellular signaling via inhibitory G proteins (Gi\/Go), modulating circadian rhythms and neuroendocrine responses independent of classical retinal photoreceptors.\u003cbr\u003e\u003cbr\u003eOPN5 is expressed in brain regions such as the hypothalamus and preoptic area, as well as in peripheral tissues including retina, cornea, skin, and reproductive organs. It functions as a UV-sensitive photoreceptor regulating local circadian oscillators and hormone secretion. In the eye, OPN5 contributes to light entrainment of retinal and non-retinal cells, while in the skin and reproductive tissues, it influences seasonal and environmental light-dependent physiology. The receptor's activation triggers G protein signaling that modulates cyclic AMP levels, gene expression, and ion channel activity.\u003cbr\u003e\u003cbr\u003eThe OPN5 antibody is a valuable tool for neurobiology, circadian rhythm, and sensory physiology research. Western blot analysis detects a 40 kilodalton band corresponding to Opsin-5, while immunofluorescence and immunohistochemistry reveal membrane localization in neuronal and epithelial tissues. This antibody supports the study of light-dependent signaling outside the visual system and enables mapping of Opsin-5 expression across tissues.\u003cbr\u003e\u003cbr\u003eDysregulation of OPN5 signaling has been linked to altered circadian rhythms, hormonal imbalances, and impaired photic entrainment. In animal models, OPN5 influences melatonin secretion and reproductive seasonality, connecting environmental lighting cues to endocrine function. The OPN5 antibody facilitates the exploration of these mechanisms by providing specific detection of Opsin-5 in experimental systems.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=OPN5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=OPN5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=OPN5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/OPN5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=OPN5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047298916717,"sku":"FY12752","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_425c1f38-6f53-4821-9e6c-31759297cf66.jpg?v=1782237045","url":"https:\/\/www.ebiohippo.com\/products\/opn5-antibody-opsin-5-neuropsin-bha17135655","provider":"BioHippo","version":"1.0","type":"link"}