{"product_id":"orc2-antibody-origin-recognition-complex-subunit-2-bha17135503","title":"ORC2 Antibody \/ Origin recognition complex subunit 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eORC2 Antibody \/ Origin recognition complex subunit 2 is a anti-ORC2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ORC2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eORC2 antibody detects Origin recognition complex subunit 2, a component of the six-subunit origin recognition complex (ORC) that initiates DNA replication. ORC2 binds replication origins throughout the genome, marking them for pre-replicative complex assembly and ensuring precise duplication of the genome each cell cycle. The ORC2 antibody is used extensively in molecular biology, replication, and cell cycle research to study DNA replication initiation and checkpoint control.\u003cbr\u003e\u003cbr\u003eORC2 is encoded by the ORC2 gene on human chromosome 2q23.1. The protein is approximately 577 amino acids long and functions as part of the core ORC complex with ORC1, ORC3, ORC4, ORC5, and ORC6. ORC2 contributes to the stability and chromatin association of the complex, anchoring it to replication origins during G1 phase. Its binding provides a platform for recruitment of CDC6, CDT1, and the MCM helicase complex required for origin licensing.\u003cbr\u003e\u003cbr\u003eThe ORC2 antibody detects a 72 kilodalton protein by western blot and demonstrates nuclear punctate staining consistent with replication foci. ORC2 remains bound to chromatin through most of the cell cycle, although its activity is regulated by phosphorylation and proteasomal turnover. Disruption of ORC2 function leads to replication defects, genomic instability, and cell cycle arrest.\u003cbr\u003e\u003cbr\u003eIn addition to replication initiation, ORC2 participates in heterochromatin organization and transcriptional silencing. ORC2 interacts with chromatin modifiers such as HP1 and histone methyltransferases to maintain heterochromatin boundaries. Deficiency of ORC2 causes chromosomal breaks and increased sensitivity to replication stress, linking replication initiation to genome integrity.\u003cbr\u003e\u003cbr\u003eBecause ORC2 is fundamental to DNA replication and chromatin architecture, it serves as a valuable marker for replication initiation studies.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ORC2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ORC2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ORC2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ORC2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ORC2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047294198125,"sku":"FY12600","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_bca75785-e358-4e3a-ae01-70306da5f808.jpg?v=1782237026","url":"https:\/\/www.ebiohippo.com\/products\/orc2-antibody-origin-recognition-complex-subunit-2-bha17135503","provider":"BioHippo","version":"1.0","type":"link"}