{"product_id":"osbpl2-antibody-oxysterol-binding-protein-like-2-bha17135896","title":"OSBPL2 Antibody \/ Oxysterol-binding protein-like 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eOSBPL2 Antibody \/ Oxysterol-binding protein-like 2 is a anti-OSBPL2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e OSBPL2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eOSBPL2 antibody detects Oxysterol-binding protein-like 2, a cytosolic lipid transfer protein that regulates cholesterol distribution, phospholipid exchange, and vesicular trafficking. The UniProt recommended name is Oxysterol-binding protein-like 2 (OSBPL2), also known as ORP2. This protein belongs to the oxysterol-binding protein (OSBP) family, which mediates non-vesicular lipid transfer and signaling between intracellular membranes.\u003cbr\u003e\u003cbr\u003eFunctionally, OSBPL2 antibody identifies a 916-amino-acid protein containing an N-terminal pleckstrin homology (PH) domain for membrane targeting and a C-terminal OSBP-related domain (ORD) that binds sterols and phosphatidylinositol phosphates. OSBPL2 functions as a lipid exchanger, shuttling cholesterol and phosphatidylserine between the endoplasmic reticulum and plasma membrane. Through this exchange, it regulates lipid signaling and membrane composition critical for endocytosis and vesicle formation.\u003cbr\u003e\u003cbr\u003eThe OSBPL2 gene is located on chromosome 20q13.12 and is expressed in a variety of tissues, including liver, brain, and muscle. OSBPL2 contributes to intracellular cholesterol homeostasis by facilitating lipid equilibration across membrane compartments. It interacts with small GTPases such as Rab7 and Arf1 to coordinate lipid trafficking with vesicular transport. Dysregulation of OSBPL2 affects lipid distribution, leading to membrane stress, endosomal dysfunction, and altered cellular signaling.\u003cbr\u003e\u003cbr\u003eIn lipid metabolism, OSBPL2 acts as a sensor for oxysterols and phosphoinositides, integrating lipid status with signaling pathways such as PI3K\/AKT and MAPK. It also modulates the activity of lipid-metabolizing enzymes and influences the localization of phosphatidylinositol 4-phosphate at membrane contact sites. Mutations in OSBPL2 are associated with autosomal dominant hearing loss, indicating its role in membrane stability and ion channel regulation in auditory hair cells.\u003cbr\u003e\u003cbr\u003eOSBPL2 antibody is widely used in membrane biology, metabolism, and vesicle trafficking research. It is suitable for immunoblotting, immunofluorescence, and subcellular fractionation studies to examine OSBPL2 localization and function. This antibody is valuable for exploring mechanisms of lipid sensing and non-vesicular transport between organelles. In metabolic and cell signaling studies, OSBPL2 antibody aids in understanding sterol regulation and membrane homeostasis.\u003cbr\u003e\u003cbr\u003eStructurally, OSBPL2 features conserved lipid-binding motifs within its ORD domain and multiple targeting sequences that direct it to membrane contact sites. It can form dimers or higher-order assemblies that facilitate lipid transfer across closely apposed membranes.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=OSBPL2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=OSBPL2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=OSBPL2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/OSBPL2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=OSBPL2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047308583277,"sku":"FY12994","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2f7a540c-9e7e-44bc-99de-64035c9c27da.jpg?v=1782237063","url":"https:\/\/www.ebiohippo.com\/products\/osbpl2-antibody-oxysterol-binding-protein-like-2-bha17135896","provider":"BioHippo","version":"1.0","type":"link"}