{"product_id":"osbpl5-antibody-oxysterol-binding-protein-related-protein-5-bha17135412","title":"OSBPL5 Antibody \/ Oxysterol-binding protein-related protein 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eOSBPL5 Antibody \/ Oxysterol-binding protein-related protein 5 is a anti-OSBPL5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e OSBPL5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eOSBPL5 antibody detects Oxysterol-binding protein-related protein 5, a lipid transfer protein involved in cholesterol and phospholipid exchange between organelles. OSBPL5 belongs to the oxysterol-binding protein (OSBP) family that mediates lipid homeostasis and endoplasmic reticulum-plasma membrane tethering. The OSBPL5 antibody is used in studies of lipid metabolism, organelle communication, and membrane dynamics.\u003cbr\u003e\u003cbr\u003eOSBPL5 is encoded by the OSBPL5 gene located on human chromosome 11q13.1. The protein is approximately 100 kilodaltons and contains a pleckstrin homology (PH) domain, a FFAT motif, and a C-terminal OSBP-like sterol-binding domain. OSBPL5 localizes to membrane contact sites between the endoplasmic reticulum and late endosomes or mitochondria, where it regulates non-vesicular lipid transport.\u003cbr\u003e\u003cbr\u003eThe OSBPL5 antibody detects a 100 kilodalton band on western blot and exhibits punctate cytoplasmic staining. Functional studies show that OSBPL5 participates in cholesterol redistribution and phosphatidylserine transport, thereby influencing membrane composition and signaling. It also regulates autophagy and mitochondrial dynamics by modulating lipid exchange at organelle interfaces.\u003cbr\u003e\u003cbr\u003eOSBPL5 plays roles in metabolism, growth regulation, and reproduction, with high expression in liver, testis, and brain. Dysregulation has been linked to metabolic disorders, including insulin resistance and obesity.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=OSBPL5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=OSBPL5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=OSBPL5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/OSBPL5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=OSBPL5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047290528109,"sku":"FY12509","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7a1e0c79-3857-46b4-92ac-5530bbc4c343.jpg?v=1782237014","url":"https:\/\/www.ebiohippo.com\/products\/osbpl5-antibody-oxysterol-binding-protein-related-protein-5-bha17135412","provider":"BioHippo","version":"1.0","type":"link"}