{"product_id":"otud3-antibody-otu-domain-containing-protein-3-bha17135702","title":"OTUD3 Antibody \/ OTU domain-containing protein 3","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eOTUD3 Antibody \/ OTU domain-containing protein 3 is a anti-OTUD3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e OTUD3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eOTUD3 antibody detects OTU domain-containing protein 3, a deubiquitinating enzyme that regulates protein stability, signaling, and cellular stress responses. Encoded by the OTUD3 gene on chromosome 1p36.13, this protein belongs to the OTU (ovarian tumor) family of cysteine proteases that cleave ubiquitin from substrate proteins, reversing ubiquitination and modulating protein degradation. OTUD3 functions as a negative regulator of the ubiquitin-proteasome system and contributes to homeostatic control of multiple signaling pathways, including p53 and AKT.\u003cbr\u003e\u003cbr\u003eStructurally, OTUD3 contains an OTU catalytic domain responsible for hydrolyzing lysine-linked ubiquitin chains, particularly K63 and K48 linkages, which regulate protein turnover and signal transduction. It stabilizes tumor suppressor p53 by removing ubiquitin moieties added by MDM2 and promotes the degradation of AKT to restrain oncogenic signaling. Through these opposing actions, OTUD3 maintains a balance between proliferation and apoptosis in response to cellular stress.\u003cbr\u003e\u003cbr\u003eThe OTUD3 antibody is used in cancer biology, proteostasis, and signal transduction research to study ubiquitin signaling, protein turnover, and stress regulation. Western blot analysis identifies a 45 kilodalton band corresponding to OTUD3, while immunofluorescence shows cytoplasmic and nuclear localization depending on cellular state. This antibody supports the investigation of deubiquitination mechanisms and ubiquitin-mediated signaling networks.\u003cbr\u003e\u003cbr\u003eAlterations in OTUD3 expression are associated with tumorigenesis, neurodegeneration, and inflammatory diseases. Overexpression of OTUD3 stabilizes p53 and enhances apoptosis, while loss of expression correlates with increased AKT activity and cancer progression. The OTUD3 antibody provides a validated tool for examining these regulatory mechanisms.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=OTUD3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=OTUD3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=OTUD3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/OTUD3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=OTUD3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047303209325,"sku":"FY12800","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_64743c83-1cee-4677-b473-3ed898b8dc16.jpg?v=1782237056","url":"https:\/\/www.ebiohippo.com\/products\/otud3-antibody-otu-domain-containing-protein-3-bha17135702","provider":"BioHippo","version":"1.0","type":"link"}