{"product_id":"ova-bhn20152448","title":"OVA","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis GenCefe mRNA encodes \u003cstrong\u003eOVA\u003c\/strong\u003e, a immunogen construct supplied for mRNA delivery tracing and transfection efficiency studies. The product is formulated as lyophilised, non-encapsulated RNA and is intended for use in cell-based research applications requiring transient protein expression with reduced immunogenicity.\u003c\/p\u003e\n\u003ch2\u003emRNA Construct Design\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003e5′ Cap:\u003c\/strong\u003e Cap1 (m7GpppNm) — co-transcriptionally added during in vitro transcription (IVT). Cap1 includes 2′-O-methylation at the first transcribed nucleotide, closely mimicking the cap structure found on endogenous mammalian mRNA and reducing recognition by innate immune sensors (e.g., IFIT1\/IFIT3).\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eModified Nucleotides:\u003c\/strong\u003e 100% N1-methylpseudouridine (m1Ψ; N1-Me-Pseudo UTP) substitution for all uridine residues. m1Ψ modification reduces TLR7\/TLR8-mediated innate immune activation and PKR-driven translational suppression, resulting in improved protein expression in immunocompetent cells and primary cell types.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003ePoly(A) Tail:\u003c\/strong\u003e 100–120 nt — enzymatically polyadenylated. The poly(A) tail stabilises the 3′ terminus, supports poly(A)-binding protein (PABP) recruitment, and enhances ribosome recycling for efficient cap-dependent translation.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003e5′ UTR:\u003c\/strong\u003e hHBA1 (hemoglobin subunit alpha 1 5′ UTR) — a well-characterised human UTR that supports efficient cap-dependent translation initiation.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003e3′ UTR:\u003c\/strong\u003e hHBA1 (hemoglobin subunit alpha 1 3′ UTR) — provides post-transcriptional stability and modulates mRNA decay kinetics.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSignal Peptide:\u003c\/strong\u003e No\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eProtein Tag:\u003c\/strong\u003e No\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCodon Optimisation:\u003c\/strong\u003e No (native human codon usage retained)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003emRNA Length:\u003c\/strong\u003e Provided upon order placement.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eForm:\u003c\/strong\u003e Lyophilised powder; reconstitute in DEPC-treated water as needed.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis mRNA is supplied as non-encapsulated, lyophilised powder. Delivery vehicle selection (LNP, electroporation, lipofection) is at the discretion of the end user and should be optimised for the target cell type and application.\u003c\/p\u003e\n\u003ch2\u003eBiological Background\u003c\/h2\u003e\n\u003cp\u003eImmunogen mRNAs encode antigenic proteins intended to stimulate immune responses in experimental models or to serve as reference antigens for antibody production and validation workflows. Ovalbumin (OVA) is a well-characterised model antigen extensively used in immunology research to study antigen presentation, T-cell activation, and tolerance induction. Keyhole limpet hemocyanin (KLH\/Hemocyanin) is a carrier protein commonly conjugated to haptens or used as an adjuvant to enhance humoral immunity. Delivering these antigens via mRNA allows dose-controlled, transient antigen expression in defined cell populations, making it compatible with therapeutic antigen-presentation studies.\u003c\/p\u003e\n\u003ch2\u003eResearch Relevance and Current Trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eTherapeutic vaccine development: mRNA immunogen platforms have demonstrated clinical proof-of-concept with approved COVID-19 vaccines; OVA-mRNA systems are widely used in preclinical immunotherapy and tolerisation studies.\u003c\/li\u003e\n  \u003cli\u003eAntigen-presenting cell transfection: mRNA-encoded antigens are used to transiently load dendritic cells for stimulation assays and adoptive transfer experiments without lentiviral safety constraints.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePreclinical antigen challenge — OVA mRNA in immunisation and tolerance induction studies in primary dendritic cells and T-cell co-culture systems.\u003c\/li\u003e\n  \u003cli\u003eAntibody production support — mRNA-based antigen expression for immunisation or cell-based antigen presentation, reducing dependence on recombinant protein purification.\u003c\/li\u003e\n  \u003cli\u003eModel antigen benchmark — OVA is a widely used reference antigen for validating new adjuvants, delivery systems, or immune modulation approaches.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes for Experimental Interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eOVA mRNA expression levels in transfected cells may not be equivalent to recombinant protein concentrations; quantify expressed antigen by ELISA or immunoblot before use in immunisation experiments.\u003c\/li\u003e\n  \u003cli\u003eImmunogenicity of mRNA-expressed antigens depends on delivery vehicle, dose, and cell type; validate antigen presentation in the intended cell system (e.g., bone marrow-derived DCs) before in vivo or functional studies.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"GenCefe Biotech","offers":[{"title":"20 ug","offer_id":53239631610221,"sku":"IR0003002","price":279.0,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53239637705069,"sku":"IR0003010","price":339.0,"currency_code":"USD","in_stock":true},{"title":"200 ug","offer_id":53239637737837,"sku":"IR0003020","price":399.0,"currency_code":"USD","in_stock":true},{"title":"500 ug","offer_id":53239637770605,"sku":"IR0003050","price":459.0,"currency_code":"USD","in_stock":true},{"title":"1 mg","offer_id":53239637803373,"sku":"IR0003100","price":549.0,"currency_code":"USD","in_stock":true},{"title":"5 mg","offer_id":53239637836141,"sku":"IR0003500","price":1799.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/mRNA_f3347e08-e781-4938-92af-3b522c59ffd1.png?v=1776677731","url":"https:\/\/www.ebiohippo.com\/products\/ova-bhn20152448","provider":"BioHippo","version":"1.0","type":"link"}