{"product_id":"oxsr1-antibody-oxidative-stress-responsive-kinase-1-bha17136223","title":"OXSR1 Antibody \/ Oxidative stress-responsive kinase 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eOXSR1 Antibody \/ Oxidative stress-responsive kinase 1 is a anti-OXSR1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunoprecipitation (IP), Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e OXSR1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IP, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eOXSR1 antibody detects Oxidative stress-responsive kinase 1, a cytoplasmic and membrane-associated serine\/threonine kinase encoded by the OXSR1 gene on chromosome 3p22.2. OXSR1 is a member of the STE20\/GC kinase family and regulates ion transport, cytoskeletal organization, and osmotic stress responses. Acting as a stress-activated kinase, OXSR1 phosphorylates cation-chloride cotransporters and cytoskeletal proteins to maintain cell volume and ionic balance during oxidative stress and osmotic challenges.\u003cbr\u003e\u003cbr\u003eActivation of OXSR1 occurs via upstream WNK1 and WNK4 kinases, which phosphorylate conserved threonine residues in its activation loop. Once active, OXSR1 phosphorylates ion transporters such as NKCC1, NKCC2, and KCC isoforms, adjusting intracellular chloride and potassium levels to sustain homeostasis. This function is vital for epithelial transport, neuronal excitability, and vascular smooth muscle contraction. OXSR1 also interacts with Rho GTPases and actin-regulating proteins to influence cytoskeletal remodeling and cell migration.\u003cbr\u003e\u003cbr\u003eIn addition to regulating ion gradients, OXSR1 participates in oxidative stress signaling, where it responds to reactive oxygen species by activating protective phosphorylation cascades. It has been shown to influence MAPK and NF-kappaB signaling pathways that mediate inflammatory responses. Through these mechanisms, OXSR1 coordinates cellular adaptation to stress conditions and maintains barrier function in epithelial and endothelial tissues. Its dual localization in the cytoplasm and at the cell membrane enables cross-talk between ion transport and signal transduction networks.\u003cbr\u003e\u003cbr\u003eDysregulation of OXSR1 contributes to disorders involving electrolyte imbalance, such as hypertension, epilepsy, and kidney disease. Mutations or abnormal activity within the WNK-OSR1-SPAK pathway can lead to altered sodium and chloride transport, impacting blood pressure regulation and renal function. Elevated OXSR1 signaling has also been linked to tumor progression and metastasis, where it promotes cell motility and invasion.\u003cbr\u003e\u003cbr\u003eStructurally, OXSR1 comprises an N-terminal kinase domain, an autoinhibitory C-terminal region, and several docking motifs that enable interactions with transporters and adaptor proteins. It shares significant homology with SPAK (STK39), its paralog in the same kinase family, and together they form a key branch of the WNK signaling pathway. OXSR1's activity integrates into the MAPK and ion transport pathways, bridging metabolic and environmental stress responses.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using OXSR1 antibody reveals cytoplasmic and plasma membrane localization in kidney, brain, and vascular smooth muscle tissues. OXSR1 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=OXSR1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=OXSR1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=OXSR1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/OXSR1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=OXSR1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047322542445,"sku":"FY13321","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5ae8601c-3bb4-4fef-8011-b4131661cef5.jpg?v=1782237112","url":"https:\/\/www.ebiohippo.com\/products\/oxsr1-antibody-oxidative-stress-responsive-kinase-1-bha17136223","provider":"BioHippo","version":"1.0","type":"link"}