{"product_id":"p-aeruginosa-dna-gyrase-bhp16300032","title":"P. aeruginosa DNA Gyrase","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eEnzyme\u003c\/span\u003e\u003cp\u003eBacterial DNA topoisomerase II (gyrase) is a type II topoisomerase that converts relaxed circular DNA into negatively supercoiled DNA. Type II topoisomerases cut and rejoin both DNA strands to change DNA topology.\u003c\/p\u003e\n\u003cp\u003eSome inhibitors, such as fluoroquinolones, block the rejoining step; the enzyme then stays covalently linked to cut DNA in a cleavage complex. Ciprofloxacin and novobiocin both inhibit gyrase, but only ciprofloxacin produces cleavage complexes.\u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctr\u003e\n\u003cth\u003e100 assays – TOP2-100PA\u003c\/th\u003e\n\u003ctd\u003e50 µl of 2 µM P. aeruginosa gyrase.\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/table\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Type\u003c\/th\u003e\n\u003ctd\u003eDNA Topoisomerases\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003ctd\u003eP. aeruginosa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e2 µM\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSizes\u003c\/th\u003e\n\u003ctd\u003e100 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog #\u003c\/th\u003e\n\u003ctd\u003eTOP2-100PA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eUse With\u003c\/h2\u003e\n\u003cp\u003eThis enzyme is used in the following ProFoldin assay kits:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/96-well-p-aeruginosa-gyrase-dna-cleavage-assay-kit-plus-bht16300064\"\u003e96-Well P. aeruginosa Gyrase DNA Cleavage Assay Kit Plus\u003c\/a\u003e (BHT16300064) — complete kit: already includes this enzyme\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/p-aeruginosa-gyrase-atpase-assay-kit-plus-bht16300070\"\u003eP. aeruginosa Gyrase ATPase Assay Kit Plus\u003c\/a\u003e (BHT16300070) — complete kit: already includes this enzyme\u003c\/li\u003e\n\u003cli\u003e\n\u003ca href=\"\/products\/p-aeruginosa-gyrase-dna-supercoiling-assay-kit-plus-bht16300071\"\u003eP. aeruginosa Gyrase DNA Supercoiling Assay Kit Plus\u003c\/a\u003e (BHT16300071) — complete kit: already includes this enzyme\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eProtocol\u003c\/h2\u003e\n\u003cp\u003eNo separate datasheet is available for this enzyme. The ProFoldin protocol “P. aeruginosa Gyrase DNA Supercoiling Assay” (linked under Documents) shows how the enzyme is used in the matching assay kit.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. This enzyme is shipped frozen. Follow standard laboratory safety practices.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"100 assays","offer_id":67815135478125,"sku":"TOP2-100PA","price":570.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/p-aeruginosa-dna-gyrase-bhp16300032","provider":"BioHippo","version":"1.0","type":"link"}