{"product_id":"p47-stable-knockout-mouse-aortic-endothelial-imaec-p47-cell-line-bhc10901383","title":"p47ᵖʰᵒˣ Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe p47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line was derived from primary mouse aortic endothelial cells isolated from the thoracic and abdominal aortas of p47phox knockout mice. The primary cells were immortalized using polyoma middle-sized T-antigen (PmT) method.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e p47ᵖʰᵒˣ Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line is supplied as an engineered cell line derived from Mouse heart.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, elongated\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS(Regular*) + 1X Endothelial Cell Growth Supplement (TM141) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in vascular biology, signaling, and assay development studies.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe p47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line is able to retain the properties and phenotype of the parental cells including the expression of common markers of endothelial cells including PECAM1, eNOS, VE-cadherin, and von Willebrand Factor. Another interesting characteristic of this cell line is the decrease in superoxide production due to the lack of p47phox. When tested for the functional responses to physiologically relevant shear stresses, such as laminar shear, the p47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line still maintained the ability to form tubes as expected from primary endothelial cells. With these characteristics, the p47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line is a useful model in cardiology, particularly in studying vascular biology and pathobiology in vitro. It is also useful in studies on p47phox and its role in superoxide production. Expression information reported for the model: PECAM1, eNOS, VE-cadherin, and von Willebrand Factor.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. PriGrow III (TM003) + 10% FBS(Regular*) + 1X Endothelial Cell Growth Supplement (TM141) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWarranty:\u003c\/strong\u003e abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period”.\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180525216109,"sku":"T3181","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/pRFSr3vuG2KgqPOWJ3F8DlP4GyU9B3HyMQaMmbqW.png?v=1774957815","url":"https:\/\/www.ebiohippo.com\/products\/p47-stable-knockout-mouse-aortic-endothelial-imaec-p47-cell-line-bhc10901383","provider":"BioHippo","version":"1.0","type":"link"}