{"product_id":"p4ha1-antibody-prolyl-4-hydroxylase-subunit-alpha-1-bha17136018","title":"P4HA1 Antibody \/ Prolyl 4-hydroxylase subunit alpha 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eP4HA1 Antibody \/ Prolyl 4-hydroxylase subunit alpha 1 is a anti-P4HA1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF) with listed reactivity in Human. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e P4HA1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eP4HA1 antibody detects Prolyl 4-hydroxylase subunit alpha 1, an enzyme that catalyzes hydroxylation of proline residues in collagen. The UniProt recommended name is Prolyl 4-hydroxylase subunit alpha 1 (P4HA1). This enzyme is essential for collagen triple-helix stabilization, extracellular matrix formation, and connective tissue integrity.\u003cbr\u003e\u003cbr\u003eFunctionally, P4HA1 antibody identifies a 534-amino-acid enzyme localized to the endoplasmic reticulum lumen, where it associates with prolyl 4-hydroxylase beta subunits to form the active tetrameric enzyme. P4HA1 catalyzes the post-translational modification of proline to 4-hydroxyproline, a critical step in collagen maturation that stabilizes the helical structure.\u003cbr\u003e\u003cbr\u003eThe P4HA1 gene is located on chromosome 10q22.1 and is expressed in fibroblasts, chondrocytes, and osteoblasts. Its expression increases during wound healing, tissue remodeling, and hypoxia. P4HA1 activity is co-regulated with other collagen synthesis enzymes and is sensitive to oxygen and ascorbate availability.\u003cbr\u003e\u003cbr\u003ePathologically, overexpression of P4HA1 has been linked to fibrosis, tumor progression, and hypoxia-induced extracellular matrix remodeling. Elevated P4HA1 enhances collagen deposition and stiffening of the tumor microenvironment, promoting invasion and metastasis. Conversely, inhibition of P4HA1 suppresses collagen accumulation and fibrotic scarring. Research using P4HA1 antibody aids studies in extracellular matrix biology, collagen synthesis, and disease-associated fibrosis.\u003cbr\u003e\u003cbr\u003eP4HA1 antibody is validated for use in western blotting, immunohistochemistry, and immunofluorescence to detect collagen-modifying enzymes. \u003cbr\u003e\u003cbr\u003eStructurally, Prolyl 4-hydroxylase subunit alpha 1 contains a catalytic dioxygenase domain that requires Fe2+ and ascorbate as cofactors for hydroxylation. This antibody enables characterization of P4HA1's role in collagen maturation, ECM formation, and disease progression.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=P4HA1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=P4HA1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=P4HA1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/P4HA1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=P4HA1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047312056685,"sku":"FY13116","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0e00b0e0-d2b5-4b56-93f9-db436cdf5ede.jpg?v=1782237094","url":"https:\/\/www.ebiohippo.com\/products\/p4ha1-antibody-prolyl-4-hydroxylase-subunit-alpha-1-bha17136018","provider":"BioHippo","version":"1.0","type":"link"}