{"product_id":"p52-relb-reporter-lentivirus-bhv19400214","title":"p52:RelB Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe p52:RelB heterodimer is the principal effector of the non-canonical (alternative) NF-κB pathway. Unlike the canonical pathway, in which p50:RelA dimers respond to stimuli such as TNFα, the non-canonical pathway is activated when ligands of the TNF receptor superfamily, including lymphotoxin-β, CD40L, BAFF, RANKL, OX40L, and TWEAK, trigger processing of the p100 precursor into p52. The resulting p52:RelB dimer enters the nucleus to activate target genes that govern lymphoid organogenesis, B-cell maturation, and immune homeostasis. Homodimers of p52 or RelB alone lack transcriptional activity, so the heterodimer provides a specific readout of non-canonical NF-κB (NF-κB2) signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe p52:RelB Reporter Lentivirus is a transcription-factor reporter system that provides a sensitive fluorescent or luminescent readout of p52:RelB heterodimer activity in mammalian cells. It uses tandem repeats of consensus p52:RelB DNA-binding elements, designed to be activated preferentially by p52:RelB heterodimers over canonical p50:RelA dimers, coupled to a minimal promoter that drives the reporter. A constitutively expressed selection marker and optional secondary reporter support stable polyclonal reporter cell lines.\u003c\/p\u003e\n\u003cp\u003eStable lentiviral integration gives consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding transient-transfection variability. Particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, supporting study of the non-canonical NF-κB2 pathway with readout by microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the p52:RelB heterodimer transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251621454189,"sku":"LTV-0105-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53362032640365,"sku":"LTV-0105-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53362032673133,"sku":"LTV-0105-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53362032705901,"sku":"LTV-0105-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53362032738669,"sku":"LTV-0105-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53362032771437,"sku":"LTV-0105-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/p52-RelB-TAG-Puro.jpg?v=1776934272","url":"https:\/\/www.ebiohippo.com\/products\/p52-relb-reporter-lentivirus-bhv19400214","provider":"BioHippo","version":"1.0","type":"link"}