{"product_id":"pabpn1-antibody-polyadenylate-binding-protein-2-bha17136362","title":"PABPN1 Antibody \/ Polyadenylate-binding protein 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003ePABPN1 Antibody \/ Polyadenylate-binding protein 2 is a anti-PABPN1 Rabbit antibody Recombinant Rabbit Monoclonal clone AAHI-16 supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Human, Mouse, Rat. Reported localization: Nucleus, Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e PABPN1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone AAHI-16, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003ePABPN1 antibody is a key reagent for studying RNA processing, gene expression, and human disease. The encoded protein, polyadenylate-binding protein nuclear 1 (also known historically as polyadenylate-binding protein 2), is a nuclear RNA-binding protein that regulates the length of the poly(A) tail added to pre-mRNA transcripts. By binding to nascent polyadenylated RNA, PABPN1 stimulates poly(A) polymerase activity and ensures proper tail length, which is critical for mRNA stability, export, and translation efficiency.\u003cbr\u003e\u003cbr\u003ePABPN1 functions primarily in the nucleus, where it coordinates with other RNA-binding proteins and processing factors to promote accurate maturation of mRNA. Beyond its role in polyadenylation, PABPN1 has been implicated in alternative polyadenylation site choice, thereby influencing transcript diversity and gene regulation. Through these activities, PABPN1 contributes to global control of gene expression and adaptation to cellular conditions.\u003cbr\u003e\u003cbr\u003eMutations in PABPN1 cause oculopharyngeal muscular dystrophy (OPMD), a late-onset genetic disorder characterized by progressive weakening of the eyelid, pharyngeal, and proximal limb muscles. The disease is associated with expansion mutations in the polyalanine tract of PABPN1, leading to nuclear aggregation of the protein and impaired RNA processing. This connection highlights the essential role of PABPN1 in muscle cell biology and its broader significance in human health.\u003cbr\u003e\u003cbr\u003eIn addition to OPMD, altered PABPN1 function has been linked to cancer and neurodegeneration, where dysregulated RNA processing contributes to disease progression. Studies suggest that changes in PABPN1 activity may affect transcriptome profiles and cellular stress responses, making it a protein of interest in both basic and translational research. Its role in regulating mRNA maturation also positions it as a critical node in pathways that integrate transcription and translation.\u003cbr\u003e\u003cbr\u003eThe PABPN1 antibody is commonly applied in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to evaluate protein expression, nuclear localization, and disease-related alterations. These applications support research into RNA metabolism, muscular dystrophy, and transcriptome regulation. For scientists investigating RNA processing, muscle pathology, or gene expression control, the PABPN1 antibody provides a reliable detection reagent.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=PABPN1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=PABPN1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=PABPN1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/PABPN1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=PABPN1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Antibody in PBS with 0.02% sodium azide, 50% glycerol and 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047326605677,"sku":"RQ8944","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f3227186-52c0-4c66-9a70-f931619cb519.jpg?v=1772019451","url":"https:\/\/www.ebiohippo.com\/products\/pabpn1-antibody-polyadenylate-binding-protein-2-bha17136362","provider":"BioHippo","version":"1.0","type":"link"}